Dioscorealide B from the traditional Thai medicine Hua-Khao-Yen induces apoptosis in MCF-7 human breast cancer cells via modulation of Bax, Bak and Bcl-2 protein expression.

Abstract

Dioscorealide B is a pharmacologically active compound from the rhizome of the Thai medicinal plant Dioscorea membranacea. Here, we demonstrated that in vitro treatment of dioscorealide B resulted in a cytotoxic effect on MCF-7 human breast cancer cells (IC50 = 2.82 microM). To determine whether this compound induces apoptosis in MCF-7, the Annexin V assay was performed. The data showed that the number of apoptotic cells were increased 7-12 folds over that of the control cells after treatment with various concentrations of dioscorealide B (3, 6 and 12 microM) for 24 hours. Dioscorealide B-induced apoptosis was associated with modulation of the multidomain Bcl-2 family members Bax, Bak and Bcl-2. After treatment with 3 microM dioscorealide B, acceleration of the level of proapoptotic proteins Bax and Bak were observed at 6 hours and 12 hours, respectively, while the decrease in the expression of antiapoptotic protein Bcl-2 was observed 3 hours after the treatment. These effects of dioscorealide B might result in the activation of caspase-8, -9 and -7, which lead to apoptosis in MCF-7 cells. Taken together, the results of this study provide evidence that dioscorealide B possesses an antitumor property against human breast cancer cells and thus provide the molecular basis for the further development of dioscorealide B as a novel chemotherapeutic agent for breast cancer treatment.

• Authors: Jiraporn Saekoo, Potchanapond Graidist, Wilairat Leeanansaksiri, Chavaboon Dechsukum, Arunporn Itharat
• Journal: Natural product communications (Nat Prod Commun) Vol. 5 Issue 12 Pg. 1921-6 (Dec 2010) ISSN: 1934-578X [Print] United States
• PMID: 21299121 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• BAK1 protein, human
• BAX protein, human
• Heterocyclic Compounds, 4 or More Rings
• Proto-Oncogene Proteins c-bcl-2
• bcl-2 Homologous Antagonist-Killer Protein
• bcl-2-Associated X Protein
• dioscorealide B
• Caspase 8

Topics

• Apoptosis (drug effects)
• Breast Neoplasms (chemistry, drug therapy, pathology)
• Caspase 8 (physiology)
• Cell Line, Tumor
• Female
• Heterocyclic Compounds, 4 or More Rings (pharmacology)
• Humans
• Plants, Medicinal (chemistry)
• Proto-Oncogene Proteins c-bcl-2 (analysis)
• Thailand
• bcl-2 Homologous Antagonist-Killer Protein (analysis)
• bcl-2-Associated X Protein (analysis)