Sperm chromatin condensation in infertile men with varicocele before and after surgical repair.

Abstract

OBJECTIVE:

To assess sperm chromatin integrity in infertile men with varicocele before and after surgical repair.

DESIGN:

Prospective.

SETTING:

Academic setting.

PATIENT(S):

Seventy-two infertile men with varicocele compared with 20 healthy fertile men.

INTERVENTION(S):

History taking, genital examination, semen analysis, sperm chromatin condensation assessment by aniline blue stain before and 3 months after varicocelectomy.

MAIN OUTCOME MEASURE(S):

Stained sperm heads (abnormal chromatin condensation) before and 3 months after varicocelectomy.

RESULT(S):

The mean percentage of aniline blue-stained sperm heads was significantly higher in infertile men with varicocele compared with fertile controls. The mean percentage of stained sperm heads was significantly decreased in infertile men with varicocele 3 months after surgery compared with the preoperative data. There was a significant negative correlation between percentage of stained sperm heads and normal morphology where nonsignificant correlation was elicited regarding sperm count and sperm motility.

CONCLUSION(S):

There is a significant increase of abnormal sperm chromatin condensation in infertile men with varicocele that is markedly improved after varicocelectomy.

• Authors: Ahmed Sadek, Abdel Shakour A Almohamdy, Amr Zaki, Mohamed Aref, Shady M Ibrahim, Taymour Mostafa
• Journal: Fertility and sterility (Fertil Steril) Vol. 95 Issue 5 Pg. 1705-8 (Apr 2011) ISSN: 1556-5653 [Electronic] United States
• PMID: 21292253 (Publication Type: Controlled Clinical Trial, Journal Article)
• Copyright: Copyright © 2011 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

Chemical References

• Chromatin

Topics

• Adult
• Chromatin (metabolism)
• Chromatin Assembly and Disassembly (physiology)
• DNA Packaging (physiology)
• Humans
• Infertility, Male (etiology, genetics, metabolism, surgery)
• Male
• Middle Aged
• Semen Analysis (methods)
• Spermatogenesis (genetics)
• Spermatozoa (metabolism)
• Urologic Surgical Procedures, Male
• Varicocele (complications, genetics, metabolism, surgery)
• Young Adult