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M cells prefer archaeosomes: an in vitro/in vivo snapshot upon oral gavage in rats.

Abstract
The archaeolipids (lipids extracted from archaebacterias) are non saponificable molecules that form self sealed mono or bilayers (archaeosomes-ARC). Different to liposomes with bilayers made of conventional glycerophospholipids, the bilayer of ARC posses a higher structural resistance to physico chemical and enzymatic degradation and surface hydrophobicity. In this work we have compared the binding capacity of ARC exclusively made of archaeols containing a minor fraction of sulphoglycophospholipids, with that of liposomes in gel phase on M-like cells in vitro. The biodistribution of the radiopharmaceutical (99m)Tc-DTPA loaded in ARC vs that of liposomes upon oral administration to Wistar rats was also determined. The fluorescence of M-like cells upon 1 and 2h incubation with ARC loaded with the hydrophobic dye Rhodamine-PE (Rh-PE) and the hydrophilic dye pyranine (HPTS) dissolved in the aqueous space, was 4 folds higher than upon incubation with equally labeled liposomes. Besides, 15% of Rh-PE and 13 % of HPTS from ARC and not from liposomes, were found in the bottom wells, a place that is equivalent to the basolateral pocket from M cells. This fact suggested the occurrence of transcytosis of ARC. Finally, 4 h upon oral administration, ARC were responsible for the 22.3 % (3.5 folds higher than liposomes) shuttling of (99m)Tc-DTPA to the blood circulation. This important amount of radioactive marker in blood could be a consequence of an extensive uptake of ARC by M cells in vivo, probably favored by their surface hydrophobicity. Taken together, these results suggested that ARC, proven their adjuvant capacity when administered by parenteral route and high biocompatibility, could be a suitable new type of nanoparticulate material that could be used as adjuvants by the oral route.
AuthorsMaria Jose Morilla, Diego Mengual Gomez, Pablo Cabral, Mirel Cabrera, Henia Balter, Maria Victoria Defain Tesoriero, Leticia Higa, Diana Roncaglia, Eder L Romero
JournalCurrent drug delivery (Curr Drug Deliv) Vol. 8 Issue 3 Pg. 320-9 (May 2011) ISSN: 1875-5704 [Electronic] United Arab Emirates
PMID21291382 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Arylsulfonates
  • Drug Carriers
  • Glyceryl Ethers
  • Liposomes
  • Rhodamines
  • archaeol lipid
  • pyranine
  • Technetium Tc 99m Pentetate
Topics
  • Administration, Oral
  • Animals
  • Archaea (chemistry)
  • Arylsulfonates (administration & dosage, chemistry)
  • Caco-2 Cells
  • Cell Line, Tumor
  • Coculture Techniques
  • Drug Carriers (administration & dosage, chemistry)
  • Glyceryl Ethers (administration & dosage, chemistry)
  • Humans
  • Hydrophobic and Hydrophilic Interactions
  • Liposomes (administration & dosage, chemistry)
  • Nanoparticles (administration & dosage, chemistry)
  • Rats
  • Rats, Wistar
  • Rhodamines (administration & dosage, chemistry)
  • Technetium Tc 99m Pentetate (administration & dosage, blood, chemistry)
  • Tissue Distribution
  • Transcytosis (physiology)

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