Human beta-defensin-1, -2, and -3 exhibit opposite effects on oral squamous cell carcinoma cell proliferation.

Abstract

The objective of this study was to investigate the impact of human beta-defensins (hBDs) on oral squamous cell carcinoma (OSCC) proliferation and hBD expression in vitro. BHY-OSCC cell lines were stimulated with hBD-1, -2, and -3. Proliferation of BHY cells was ascertained and hBD-mRNA expression was evaluated by real-time PCR. Proliferation of BHY cells decreased by 25% in response to hBD-1 stimulation but increased after stimulation with hBD-2 and -3. HBD-1 stimulation enhanced hBD-3 expression, whereas HBD-2 stimulation decreased early hBD-3 expression. HBD-3 stimulation enhanced hBD-1 expression. HBDs profoundly impact on OSCC proliferation and hBD expression in vitro. Therefore, hBD-1 might function as a tumor suppressor gene in OSCCs, while hBD-2 and -3 might be protooncogenes.

• Authors: Jochen Winter, Annette Pantelis, Rudolf Reich, Markus Martini, Dominik Kraus, Soeren Jepsen, Jean-Pierre Allam, Natalija Novak, Matthias Wenghoefer
• Journal: Cancer investigation (Cancer Invest) Vol. 29 Issue 3 Pg. 196-201 (Mar 2011) ISSN: 1532-4192 [Electronic] England
• PMID: 21280982 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DEFB1 protein, human
• DEFB103A protein, human
• DEFB4A protein, human
• RNA, Messenger
• beta-Defensins

Topics

• Carcinoma, Squamous Cell (genetics, metabolism, pathology)
• Cell Line, Tumor
• Cell Proliferation
• Humans
• Mouth Neoplasms (genetics, metabolism, pathology)
• RNA, Messenger (analysis)
• Reverse Transcriptase Polymerase Chain Reaction
• beta-Defensins (metabolism)