Abstract |
Many biologically active agents exert a pleiotropic response in cells and tissues. This presents challenges in descriptive and comparative analysis of the proteome in response to these agents. Although free-flow electrophoresis has been applied in a number of proteomic studies as a protein separation technique, the combination of free-flow electrophoresis and DIGE has not yet been investigated for comparative proteomic analysis. In this study, we have compared the effects of butyrate on HT29 colorectal cancer cells with a particular focus on apoptosis and describe the utility of a novel approach combining free-flow electrophoresis with DIGE to identify differentially expressed proteins. We verify the results obtained by the combined free-flow electrophoresis and DIGE approach with Western blot analysis of selected proteins. We also report for the first time the regulation of a number of proteins by butyrate in HT29 colorectal cells including peptidyl-prolyl cis-trans isomerase A ( cyclophilin A) and profilin-1.
|
Authors | Kim Y C Fung, Chris Cursaro, Tanya Lewanowitsch, Gemma V Brierley, Shaun R McColl, Trevor Lockett, Richard Head, Peter Hoffmann, Leah Cosgrove |
Journal | Proteomics
(Proteomics)
Vol. 11
Issue 5
Pg. 964-71
(Mar 2011)
ISSN: 1615-9861 [Electronic] Germany |
PMID | 21280223
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Chemical References |
- Butyrates
- Profilins
- Protective Agents
- Proteome
- Cyclophilin A
|
Topics |
- Apoptosis
(drug effects)
- Blotting, Western
- Butyrates
(pharmacology)
- Cell Line, Tumor
- Colorectal Neoplasms
(genetics, metabolism, prevention & control)
- Cyclophilin A
(genetics, metabolism)
- Gene Expression Profiling
- Gene Expression Regulation, Neoplastic
(drug effects)
- HT29 Cells
- Humans
- Profilins
(genetics, metabolism)
- Protective Agents
(pharmacology)
- Proteome
(genetics, metabolism)
- Proteomics
(methods)
- Two-Dimensional Difference Gel Electrophoresis
|