Myocardin-related transcription factor-A induces cardiomyocyte hypertrophy.

Abstract

Myocardin is a remarkably potent transcriptional coactivator expressed specifically in cardiac muscle lineages and smooth muscle cells during postnatal development. Myocardin shares homology with myocardin-related transcription factor-A (MRTF-A), which are expressed in a broad range of embryonic and adult tissues. Our previous results show that myocardin induces cardiac hypertrophy. However, the effects of MRTF-A in cardiac hypertrophy remain poorly understood. Our present work further demonstrates that myocardin plays an important role in inducing hypertrophy. At the same time, we find that overexpression of MRTF-A in neonatal rat cardiomyocytes might induce cardiomyocyte hypertrophy. Furthermore, MRTF-A expression is induced in phenylephrine, angiotensin-II, and transforming growth factor-β-stimulated cardiac hypertrophy, whereas a dominant-negative form of MRTF-A or MRTF-A siRNA strongly inhibited upregulation of hypertrophy genes in response to hypertrophic agonists in neonatal rat cardiomyocytes. Our studies indicate that besides myocardin, MRTF-A might play an important role in cardiac hypertrophy. Our findings provide novel evidence for the future studies to explore the roles of MRTFs in cardiac hypertrophy.

• Authors: Xing-Hua Liao, Nan Wang, Qing-Xi Liu, Tao Qin, Bo Cao, Dong-Sun Cao, Tong-Cun Zhang
• Journal: IUBMB life (IUBMB Life) Vol. 63 Issue 1 Pg. 54-61 (Jan 2011) ISSN: 1521-6551 [Electronic] England
• PMID: 21280178 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2010 Wiley Periodicals, Inc.

Chemical References

• DNA Primers
• Transcription Factors
• myocardin-related transcription factor-A, rat

Topics

• Animals
• Base Sequence
• Blotting, Western
• DNA Primers
• Immunohistochemistry
• Myocardium (pathology)
• Rats
• Rats, Sprague-Dawley
• Reverse Transcriptase Polymerase Chain Reaction
• Transcription Factors (metabolism)