Abstract | BACKGROUND: METHODS: Study was conducted on 226 newborns. Hematological parameters were recorded and Hb profiles were examined on the Capillarys 2 Hemoglobin analyzer (SEBIA). DNA analyses were used to establish the final diagnoses. RESULTS: Among 226 newborns examined, 122 had thalassemias with 17 different genotypes. The capillary electrophoresis system could provide useful data for presumptive diagnoses of cases, especially those with Hb E and α- thalassemia. Hb E was found to be 2.6-6.2% in heterozygote whereas Hb Bart's were clearly observed in cases with compound heterozygous or homozygous α(+)- thalassemia and heterozygous α(0)-thalassemia. Hb H disease and other forms of α- thalassemia could be differentiated based on the presence of Hb Bart's and its percentage. CONCLUSION: The capillary electrophoresis system is applicable to newborn screening for common forms of thalassemia in Southeast Asia.
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Authors | Hataichanok Srivorakun, Goonnapa Fucharoen, Yossombat Changtrakul, Patcharee Komwilaisak, Supan Fucharoen |
Journal | Clinical biochemistry
(Clin Biochem)
Vol. 44
Issue 5-6
Pg. 406-11
(Apr 2011)
ISSN: 1873-2933 [Electronic] United States |
PMID | 21277293
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved. |
Topics |
- Asia, Southeastern
- Electrophoresis, Capillary
(methods)
- Hemoglobinopathies
(diagnosis, genetics)
- Humans
- Infant, Newborn
- Neonatal Screening
- Thalassemia
(diagnosis, genetics)
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