Abstract |
Gram-negative bacterial outer membrane proteins (Omps) have an important role in pathogenesis and signal reception. We previously reported that Acinetobacter OmpA (AbOmpA) induced maturation of bone marrow-derived dendritic cells (BMDCs) and that AbOmpA-primed DCs produced IL-12 which generated Th1 CD4(+) T-cells. We analyzed the effects of Salmonella typhimurium OmpA (OmpA-Sal) on dendritic cell (DC) maturation in the present study, and determined that tumor antigen-pulsed DCs stimulated with OmpA-Sal induced anti- tumor responses in a mouse model. OmpA-Sal activated BMDCs by augmenting expression of MHC class II and of the co-stimulatory molecules CD80 and CD86. RT-PCR revealed that IL-12(p40) gene expression is highly augmented in OmpA-Sal-stimulated BMDCs. DNA (CRT/E7) vaccination combined with OmpA-Sal stimulation generated more antigen-specific CD8(+) T-cells in the present study. Certain antigen-pulsed BMDCs stimulated with OmpA-Sal induced strong PADRE-specific CD4(+) and E7-specific CD8(+) T-cell responses. In addition, BMDCs stimulated with OmpA-Sal (OmpA-Sal-BMDCs) and pulsed with both E7 and PADRE peptide generated greater numbers of E7-specific CD8(+) effector and memory T-cells than those pulsed with E7 peptide alone. E7- and PADRE-expressing OmpA-Sal-BMDC vaccines resulted in significant long-term protective anti- tumor effects in vaccinated mice. Our data suggested that E7- and PADRE-expressing BMDCs that were matured in the presence of OmpA-Sal might enhance anti- tumor immunity and support the therapeutic use of OmpA-Sal in DC-based immunotherapy.
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Authors | Min Ja Jang, Jee-Eun Kim, Yoon Hee Chung, Won Bok Lee, Yong Kyoo Shin, Jun Sik Lee, Daejin Kim, Yeong-Min Park |
Journal | Vaccine
(Vaccine)
Vol. 29
Issue 13
Pg. 2400-10
(Mar 16 2011)
ISSN: 1873-2518 [Electronic] Netherlands |
PMID | 21276440
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 Elsevier Ltd. All rights reserved. |
Chemical References |
- Antigens, Neoplasm
- Antigens, Surface
- Bacterial Outer Membrane Proteins
- Cancer Vaccines
- Cytokines
- OMPA outer membrane proteins
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Topics |
- Animals
- Antigens, Neoplasm
(immunology)
- Antigens, Surface
(analysis)
- Bacterial Outer Membrane Proteins
(immunology)
- CD4-Positive T-Lymphocytes
(immunology)
- CD8-Positive T-Lymphocytes
(immunology)
- Cancer Vaccines
(immunology)
- Cytokines
(biosynthesis)
- Dendritic Cells
(chemistry, immunology)
- Female
- Gene Expression Profiling
- Mice
- Mice, Inbred C57BL
- Neoplasms
(prevention & control)
- Reverse Transcriptase Polymerase Chain Reaction
- Salmonella typhimurium
(immunology)
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