[Effect of cinobufotalin on growth of xenograft of endometrial carcinoma cell line ishikawa in nude mouse and its impact on RRM2 expression].

Abstract

OBJECTIVE:

To investigate the inhibitory effect of cinobufotalin (CBT) on the growth of xenograft endometrial carcinoma cell line ishikawa in nude mice, and its impact on the expression of ribonucleotide reductase subunit M2 (RRM2).

METHODS:

Eleven nude mice with xenograft were randomly divided into two groups, the CBT group and the control group, which received intra-tumor injection of CBT and saline respectively for one week. The sizes of xenografts were measured before and after the treatment to calculate the inhibition ratio of tumor proliferation; the RRM2-mRNA and protein expressions in tumor tissue were measured by RT-PCR and Western blot respectively.

RESULTS:

After treatment, the size of xenografts in the CBT group was (0.1314 +/- 0.0304) cm3, which was significantly lower than that in the control group (0.360 0 +/- 0.1145) cm3, (P < 0.05), the tumor proliferation inhibition ratio being 43.46%. The differences of RRM2 mRNA and protein expression levels between the two group were significant (P = 0.019 and P = 0.001).

CONCLUSION:

CBT significantly inhibits the growth of the xenografts of endometrial carcinoma Ishikawa in nude mice, and the action mechanism is possibly associated with the inhibition on RRM2 expression.

• Authors: Kun Feng, Huai-Jun Zhou, Ya-Li Hu
• Journal: Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine (Zhongguo Zhong Xi Yi Jie He Za Zhi) Vol. 30 Issue 11 Pg. 1183-5 (Nov 2010) ISSN: 1003-5370 [Print] China
• PMID: 21275172 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Bufanolides
• Materia Medica
• RNA, Messenger
• ribonucleotide reductase M2
• Ribonucleoside Diphosphate Reductase
• cinobufotalin

Topics

• Animals
• Antineoplastic Agents (pharmacology)
• Bufanolides (pharmacology)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Endometrial Neoplasms (genetics, metabolism, pathology)
• Female
• Humans
• Materia Medica (pharmacology)
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• RNA, Messenger (genetics, metabolism)
• Ribonucleoside Diphosphate Reductase (genetics, metabolism)
• Xenograft Model Antitumor Assays