Abstract | OBJECTIVE: METHODS: Eleven nude mice with xenograft were randomly divided into two groups, the CBT group and the control group, which received intra- tumor injection of CBT and saline respectively for one week. The sizes of xenografts were measured before and after the treatment to calculate the inhibition ratio of tumor proliferation; the RRM2-mRNA and protein expressions in tumor tissue were measured by RT-PCR and Western blot respectively. RESULTS:
After treatment, the size of xenografts in the CBT group was (0.1314 +/- 0.0304) cm3, which was significantly lower than that in the control group (0.360 0 +/- 0.1145) cm3, (P < 0.05), the tumor proliferation inhibition ratio being 43.46%. The differences of RRM2 mRNA and protein expression levels between the two group were significant (P = 0.019 and P = 0.001). CONCLUSION: CBT significantly inhibits the growth of the xenografts of endometrial carcinoma Ishikawa in nude mice, and the action mechanism is possibly associated with the inhibition on RRM2 expression.
|
Authors | Kun Feng, Huai-Jun Zhou, Ya-Li Hu |
Journal | Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine
(Zhongguo Zhong Xi Yi Jie He Za Zhi)
Vol. 30
Issue 11
Pg. 1183-5
(Nov 2010)
ISSN: 1003-5370 [Print] China |
PMID | 21275172
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Antineoplastic Agents
- Bufanolides
- Materia Medica
- RNA, Messenger
- ribonucleotide reductase M2
- Ribonucleoside Diphosphate Reductase
- cinobufotalin
|
Topics |
- Animals
- Antineoplastic Agents
(pharmacology)
- Bufanolides
(pharmacology)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Endometrial Neoplasms
(genetics, metabolism, pathology)
- Female
- Humans
- Materia Medica
(pharmacology)
- Mice
- Mice, Inbred BALB C
- Mice, Nude
- RNA, Messenger
(genetics, metabolism)
- Ribonucleoside Diphosphate Reductase
(genetics, metabolism)
- Xenograft Model Antitumor Assays
|