Anticancer and antimetastatic activities of Renieramycin M, a marine tetrahydroisoquinoline alkaloid, in human non-small cell lung cancer cells.

Abstract

BACKGROUND:

Renieramycin M, has been shown to exhibit promising anticancer activity against some cancer cell lines; however, the underlying mechanism remains unknown.

MATERIALS AND METHODS:

Renieramycin M was isolated from the blue sponge Xestospongia sp. Anticancer and antimetastatic activities of renieramycin M were investigated in human non-small cell lung cancer cells.

RESULTS:

Renieramycin M treatment caused p53 activation, which subsequently down-regulated anti-apoptotic MCL-1 and BCL-2 proteins, while the level of pro-apoptotic BAX protein was not altered. The subtoxic concentrations of renieramycin M significantly decreased invasion and migration abilities of cancer cells. In addition, this compound showed a strong inhibitory effect on anchorage-independent growth of the cells.

CONCLUSION:

These results reveal that renieramycin M induced lung cancer cells apoptosis through p53-dependent pathway and the compound may inhibit progression and metastasis of lung cancer cells.

• Authors: Hasseri Halim, Preedakorn Chunhacha, Khanit Suwanborirux, Pithi Chanvorachote
• Journal: Anticancer research (Anticancer Res) Vol. 31 Issue 1 Pg. 193-201 (Jan 2011) ISSN: 1791-7530 [Electronic] Greece
• PMID: 21273598 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Alkaloids
• Myeloid Cell Leukemia Sequence 1 Protein
• Proto-Oncogene Proteins c-bcl-2
• TP53 protein, human
• Tetrahydroisoquinolines
• Tumor Suppressor Protein p53
• renieramycin M

Topics

• Alkaloids (pharmacology)
• Animals
• Anoikis (drug effects)
• Apoptosis (drug effects)
• Blotting, Western
• Carcinoma, Non-Small-Cell Lung (drug therapy, metabolism, pathology)
• Cell Adhesion (drug effects)
• Cell Movement (drug effects)
• Cell Proliferation (drug effects)
• Colony-Forming Units Assay
• Humans
• Lung Neoplasms (drug therapy, metabolism, pathology)
• Myeloid Cell Leukemia Sequence 1 Protein
• Porifera (chemistry)
• Proto-Oncogene Proteins c-bcl-2 (metabolism)
• Tetrahydroisoquinolines (pharmacology)
• Tumor Cells, Cultured
• Tumor Suppressor Protein p53 (metabolism)
• Wound Healing (drug effects)