Abstract |
The role of CD4(+) T cells in bladder autoimmune inflammation has not been identified because of the lack of a proper animal model. We investigated CD4(+) T-cell responses to bladder urothelial ovalbumin (OVA), a model self-antigen (Ag), in transgenic URO-OVA mice. The expression of bladder urothelial OVA rendered mice unresponsive to OVA and resulted in quick clearance of Ag-specific CD4(+) T cells. Adoptive transfer of naive OVA-specific CD4(+) T cells led to exogenous T-cell proliferation, activation, and bladder infiltration but no inflammatory induction. In contrast, adoptive transfer of preactivated OVA-specific CD4(+) T cells induced bladder inflammation. Studies further demonstrated that CD4(+) T cells induced bladder inflammation in URO-OVA mice depleted of CD8(+) T cells or deficient in the recombinase activating gene-1 (Rag-1(-/-)). These results indicate that urothelial Ag-specific CD4(+) T cells can function as direct effector cells to induce bladder autoimmune inflammation independent of CD8(+) T cells.
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Authors | W Liu, X Chen, D P Evanoff, Y Luo |
Journal | Mucosal immunology
(Mucosal Immunol)
Vol. 4
Issue 4
Pg. 428-37
(Jul 2011)
ISSN: 1935-3456 [Electronic] United States |
PMID | 21270773
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
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Topics |
- Adoptive Transfer
- Animals
- Autoimmune Diseases
(immunology, pathology)
- Autoimmunity
(immunology)
- CD4-Positive T-Lymphocytes
(immunology)
- CD8-Positive T-Lymphocytes
(immunology)
- Cystitis
(immunology, pathology)
- Immune Tolerance
(immunology)
- Lymphocyte Activation
(immunology)
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Ovalbumin
(immunology)
- Urothelium
(immunology, pathology)
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