Abstract | AIM: METHODS: We incubated RAW264.7 cells (RAW) and mouse peritoneal macrophages ( MPMs) with sitosterol (Sito), campesterol (Camp) or cholesterol (Chol) at low (8 µM, 16 µM) or high (160 µM) concentrations, and investigated their effects on LPS-induced secretion of IL-6 and TNF-α. We also analyzed their effects on endoplasmic reticulum (ER) stress in both cells, and on the cell proliferation of RAW. RESULTS: At low sterol concentrations, only Chol resulted in a tendency toward the increased secretion of TNF-α from MPMs. At high concentrations, Chol induced a significant increase in TNF-α secretions from both cells; however, Sito resulted in a non-significant increase in TNF-α secretion. The effects on IL-6 secretions of Sito were also significantly less than those of Chol. Camp increased the secretions of both cytokines from MPMs; however, the extent of these increases was less pronounced than that of Chol. Augmentation of ER stress was greatest with Chol among the sterols, and the proliferation of RAW cells was inhibited only with Chol. CONCLUSION:
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Authors | Makoto Kurano, Naoyuki Iso-O, Masumi Hara, Eisei Noiri, Kazuhiko Koike, Takashi Kadowaki, Kazuhisa Tsukamoto |
Journal | Journal of atherosclerosis and thrombosis
(J Atheroscler Thromb)
Vol. 18
Issue 5
Pg. 373-83
( 2011)
ISSN: 1880-3873 [Electronic] Japan |
PMID | 21266789
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- DNA-Binding Proteins
- Interleukin-6
- Lipopolysaccharides
- Phytosterols
- Regulatory Factor X Transcription Factors
- Transcription Factors
- Tumor Necrosis Factor-alpha
- campesterol
- Cholesterol
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Topics |
- Alternative Splicing
- Animals
- Cell Proliferation
(drug effects)
- Cells, Cultured
- Cholesterol
(analogs & derivatives, pharmacology)
- DNA-Binding Proteins
(genetics)
- Endoplasmic Reticulum
(drug effects, metabolism)
- Hypercholesterolemia
- Interleukin-6
(metabolism)
- Intestinal Diseases
- Lipid Metabolism, Inborn Errors
- Lipopolysaccharides
(pharmacology)
- Macrophage Activation
(drug effects)
- Macrophages
(drug effects, metabolism)
- Mice
- Phytosterols
(adverse effects, pharmacology)
- Regulatory Factor X Transcription Factors
- Transcription Factors
(genetics)
- Tumor Necrosis Factor-alpha
(metabolism)
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