Artepillin C (3,5-diprenyl-p-coumaric acid), a major compound found in Brazilian green
propolis and Baccharis dracunculifolia, shows anti-inflammatory, antibacterial,
antiviral,
antioxidant and antitumoral activities, among others. The aim of this study was to evaluate the genotoxic potential of
artepillin C and its ability to prevent the chemically induced
chromosome breakage or loss and the primary DNA damage using the micronucleus and comet assays in male Swiss mice, respectively. The animals were treated by gavage with different doses of
artepillin C (0.4, 0.8 and 1.6 mg kg(-1) b.w.). For the antigenotoxicity assays, the different doses of
artepillin C were administered simultaneously to
doxorubicin (DXR; micronucleus test; 15 mg kg(-1) b.w.) and to
methyl methanesulfonate (MMS; comet assay; 40 mg kg(-1) b.w.). The results showed that
artepillin C itself was not genotoxic in the mouse micronucleus and comet assays. In the animals treated with
artepillin C and DXR, the number of micronucleated reticulocytes was significantly lower in comparison with the animals treated only with DXR. Regarding antigenotoxicity,
artepillin C at the tested doses significantly reduced the extent of DNA damage in liver cells induced by MMS.