Human MxA protein inhibits the replication of classical swine fever virus.

Abstract

Classical swine fever virus (CSFV) has a spherical enveloped particle with a single stranded RNA genome, the virus belonging to a pestivirus of the family Flaviviridae is the causative agent of an acute contagious disease classical swine fever (CSF). The interferon-induced MxA protein has been widely shown to inhibit the life cycle of certain RNA viruses as members of the Bunyaviridae family and others. Interestingly, it has been reported that expression of MxA in infected cells was blocked by CSFV and whether MxA has an inhibitory effect against CSFV remains unknown to date until present. Here, we report that CSFV replicated poorly in cells stably transfected with human MxA. The proliferation of progeny virus in both PK-15 cell lines and swine fetal fibroblasts (PEF) continuously expressing MxA was shown significantly inhibited as measured by virus titration, indirect immune fluorescence assay and real-time PCR.

• Authors: Yicheng Zhao, Daxin Pang, Tiedong Wang, Xin Yang, Rong Wu, Linzhu Ren, Ting Yuan, Yongye Huang, Hongsheng Ouyang
• Journal: Virus research (Virus Res) Vol. 156 Issue 1-2 Pg. 151-5 (Mar 2011) ISSN: 1872-7492 [Electronic] Netherlands
• PMID: 21255621 (Publication Type: Journal Article)
• Copyright: Copyright © 2011 Elsevier B.V. All rights reserved.

Chemical References

• MX1 protein, human
• Myxovirus Resistance Proteins
• Recombinant Proteins
• GTP-Binding Proteins

Topics

• Animals
• Cell Line
• Classical Swine Fever (virology)
• Classical Swine Fever Virus (physiology)
• GTP-Binding Proteins (genetics, metabolism)
• Gene Dosage
• Gene Order
• Humans
• Myxovirus Resistance Proteins
• Recombinant Proteins (genetics, metabolism)
• Restriction Mapping
• Swine
• Viral Load
• Virus Replication (physiology)