Abstract | ETHNOPHARMACOLOGICAL RELEVANCE: The rhizomes of Kaempferia parviflora Wall. ex Baker have been traditionally used in Thailand to treat abscesses, gout, and peptic ulcers. AIM: MATERIALS AND METHODS: The chloroform fraction was isolated using silica gel column chromatography and HPLC. Isolated compounds were tested against NO and TNF-α using RAW264.7 cells. Cytotoxicity and iNOS, p-ERK and p-JNK expression were also examined. RESULTS: Three active components, 5,7-dimethoxyflavone (2), trimethylapigenin (4), and tetramethylluteolin (5), markedly inhibited the production of NO in lipopolysaccharide (LPS)-activated RAW264.7 cells. Compounds 2, 4, and 5 moderately inhibited production of TNF-α. Compounds 2, 4, and 5 strongly inhibited expression of iNOS mRNA and iNOS protein in a dose-dependent manner, but did not inhibit p-ERK or p-JNK protein expression. The most active compound, 4, did not inhibit the enzyme activity of inhibitor of κB kinases or mitogen-activated protein kinases, but inhibited that of spleen tyrosine kinase (SYK). CONCLUSION: The mechanism responsible for the anti-inflammatory activity of methoxyflavonoids from the chloroform fraction of the rhizomes of Kaempferia parviflora is mainly the inhibition of iNOS expression, and the inhibition of SYK by 4 may be involved in the suppression of LPS-induced signaling in macrophages.
|
Authors | Chutha Sae-Wong, Hisashi Matsuda, Supinya Tewtrakul, Pimpimon Tansakul, Seikou Nakamura, Yukiko Nomura, Masayuki Yoshikawa |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 136
Issue 3
Pg. 488-95
(Jul 14 2011)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 21251970
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright © 2011 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Anti-Inflammatory Agents
- Flavones
- Flavonoids
- Intracellular Signaling Peptides and Proteins
- Lipopolysaccharides
- Plant Extracts
- RNA, Messenger
- Tumor Necrosis Factor-alpha
- 5,7-dimethoxyflavone
- Nitric Oxide
- Apigenin
- Nitric Oxide Synthase Type II
- Protein-Tyrosine Kinases
- Syk Kinase
- Syk protein, mouse
- Syk protein, rat
- Extracellular Signal-Regulated MAP Kinases
- JNK Mitogen-Activated Protein Kinases
- Luteolin
|
Topics |
- Animals
- Anti-Inflammatory Agents
(isolation & purification, pharmacology, therapeutic use)
- Apigenin
(isolation & purification, pharmacology, therapeutic use)
- Cell Line
- Dose-Response Relationship, Drug
- Extracellular Signal-Regulated MAP Kinases
(metabolism)
- Flavones
(isolation & purification, pharmacology, therapeutic use)
- Flavonoids
(isolation & purification, pharmacology, therapeutic use)
- Inflammation
(drug therapy, metabolism)
- Intracellular Signaling Peptides and Proteins
(antagonists & inhibitors)
- JNK Mitogen-Activated Protein Kinases
(metabolism)
- Lipopolysaccharides
- Luteolin
(isolation & purification, pharmacology, therapeutic use)
- Macrophages
(drug effects, metabolism)
- Mice
- Nitric Oxide
(biosynthesis)
- Nitric Oxide Synthase Type II
(genetics, metabolism)
- Phytotherapy
- Plant Extracts
(chemistry, pharmacology, therapeutic use)
- Protein-Tyrosine Kinases
(antagonists & inhibitors)
- RNA, Messenger
(metabolism)
- Rhizome
- Syk Kinase
- Tumor Necrosis Factor-alpha
(metabolism)
- Zingiberaceae
(chemistry)
|