Changes of CCAAT enhancer-binding proteins (CEBPs) in the lung of streptozotocin-induced diabetic rats.

CCAAT enhancer-binding proteins (CEBPs) play key roles in the metabolic regulation, cell transformation, and inflammation. However, the expression and/or functions of CEBPs in rats with hyperglycemia are still unclear. In the present study, we investigated the changes of CEBPs protein in lung of the diabetic rats. The levels of C/EBPβ and C/EBPδ protein were decreased in the lung isolated from streptozotocin-induced diabetic rats (STZ-diabetic rats) as compared with that of normal rats. Exogenous insulin at the dose sufficient to normalize the plasma glucose of STZ-diabetic rats reversed the protein levels of C/EBPβ and C/EBPδ in lung after a 4-day treatment. Similar results were also observed in STZ-diabetic rats that received the treatment of phlorizin to reverse the plasma glucose level for 4 days. Otherwise, the protein level of C/EBPα in lung of the STZ-diabetic rats was similar as the normal rats. Also, the level of C/EBPα protein in lung of the STZ-diabetic rat was not significantly changed by correction of plasma glucose by exogenous insulin or phlorizin. In addition, we also cultured human lung cells (A-549) and rat lung cells (L2) in varies concentration of D-glucose and L-glucose to identify the effect of glucose in expression of C/EBPs. The obtained results suggest that increase of plasma glucose is related to the lower expression of C/EBPβ and C/EBPδ proteins in the lung of STZ-diabetic rats. The changes of expression of C/EBPβ and C/EBPδ are not caused by changes of osmolarity but by D-glucose itself.
AuthorsY Fong, K-H Shen, L-J Chen, J-T Cheng
JournalHormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et métabolisme (Horm Metab Res) Vol. 43 Issue 4 Pg. 261-7 (Apr 2011) ISSN: 1439-4286 [Electronic] Germany
PMID21249617 (Publication Type: Journal Article)
Copyright© Georg Thieme Verlag KG Stuttgart · New York.
Chemical References
  • CCAAT-Enhancer-Binding Protein-alpha
  • CCAAT-Enhancer-Binding Protein-beta
  • CCAAT-Enhancer-Binding Protein-delta
  • Streptozocin
  • Phlorhizin
  • Glucose
  • Animals
  • CCAAT-Enhancer-Binding Protein-alpha (genetics, metabolism)
  • CCAAT-Enhancer-Binding Protein-beta (genetics, metabolism)
  • CCAAT-Enhancer-Binding Protein-delta (genetics, metabolism)
  • Diabetes Mellitus, Experimental (drug therapy, genetics, metabolism)
  • Glucose (metabolism)
  • Humans
  • Lung (metabolism)
  • Male
  • Phlorhizin (therapeutic use)
  • Rats
  • Rats, Wistar
  • Streptozocin

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