Abstract |
Bombyx mori densonucleosis virus (BmDNV) is one of the most disastrous viruses in cocoon production. Silkworm resistance to BmDNV has been examined previously using a number of traditional biochemical and molecular techniques. In this study, a near isogenic line, BC(6), was constructed to eliminate the difference in inherited background, which has 99.9% identity with the susceptible strain but carries a resistant gene. We utilized a proteomic approach involving two-dimensional differential gel electrophoresis and mass spectrometry to examine changes in the midgut proteins from the susceptible and resistant silkworm larvae infected with BmDNV. The protein profiles were compared and 9 differentially expressed proteins were identified by mass spectrometry. In the resistant strains, the heat-shock 70-kDa protein cognate, cytochrome P450, vacuolar ATP synthase subunit B, arginine kinase, vacuolar ATP synthase subunit D and glutathione S-transferase sigma were strongly upregulated and α-tubulin was downregulated. Our results imply that these upregulated genes and the downregulated genes might be involved in B. mori immune responses against BmDNV-Z infection.
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Authors | Hui-Qing Chen, Qin Yao, Fang Bao, Ke-Ping Chen, Xiao-Yong Liu, Jun Li, Lin Wang |
Journal | Intervirology
(Intervirology)
Vol. 55
Issue 1
Pg. 21-8
( 2012)
ISSN: 1423-0100 [Electronic] Switzerland |
PMID | 21242662
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 S. Karger AG, Basel. |
Chemical References |
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Topics |
- Animals
- Bombyx
(chemistry, immunology, virology)
- Densovirinae
(immunology, pathogenicity)
- Electrophoresis, Gel, Two-Dimensional
- Gastrointestinal Tract
(chemistry, immunology, virology)
- Gene Expression Profiling
- Mass Spectrometry
- Proteome
(analysis)
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