Mammary tumor immunoscintigraphy in rats: the use of 131I-anti-estriol 3-sulfate antibody.

Abstract

The scintigraphic imaging of mammary tumors with anti-estriol 3-sulfate (E3 3-S) antibody was studied in rats. A chemical carcinogen, 7,12-dimethylbenz(a)anthracine (DMBA), induced mammary tumors in Sprague-Dawley female rats. Highly specific anti-E3 3-S antibody was prepared and radioiodinated by [131I]NaI using the chloramine-T method. At 24 h after administration of 131I-anti-E3 3-S antibody, goat anti-guinea pig immunogloblin G (IgG) was injected as the second antibody (SA) and nuclear scintigraphy was performed. Mammary tumors were clearly visualized following SA injection.

• Authors: N Shimura, Y Komori, T Tanaka, A Kubodera, H Oyamada
• Journal: International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology (Int J Rad Appl Instrum B) Vol. 17 Issue 6 Pg. 553-60 ( 1990) ISSN: 0883-2897 [Print] England
• PMID: 2123840 (Publication Type: Journal Article)

Chemical References

• Antibodies
• Immunoglobulin G
• Iodine Radioisotopes
• estriol 3-sulfate
• 9,10-Dimethyl-1,2-benzanthracene
• Estriol

Topics

• 9,10-Dimethyl-1,2-benzanthracene
• Animals
• Antibodies (pharmacokinetics)
• Estriol (immunology)
• Female
• Goats (immunology)
• Guinea Pigs
• Immunoglobulin G (administration & dosage)
• Iodine Radioisotopes
• Mammary Neoplasms, Experimental (chemically induced, diagnostic imaging, immunology)
• Radionuclide Imaging
• Rats
• Rats, Inbred Strains
• Tissue Distribution