Abstract |
The soluble hemagglutinin/protease ( HA/protease) produced by Vibrio cholerae and the elastase of Pseudomonas aeruginosa are both zinc/ calcium-dependent proteases. In the present study the two enzymes are compared immunologically and functionally. The N-terminal amino acid sequences of the proteins had 65% identity within the first 20 amino acids. Polyclonal antisera against each purified protein recognized the enzyme of the other species in enzyme-linked immunosorbent assay, checkerboard immunoblot, and Western blot analyses and inhibited the protease activity of both enzymes in milk and elastin agars. Like the HA/protease, the elastase hemagglutinated "responder" but not "nonresponder" chicken erythrocytes, degraded ovomucin, lactoferrin, and fibronectin, and nicked the A subunit of the cholera toxin-related heat-labile enterotoxin from Escherichia coli. Whereas none of the three proteases tested ( elastase, HA/protease, or pronase E) had any obvious effect in ileal loop tests in rabbits at doses up to 50 micrograms, all three produced some detectable skin reactions at a dose of 0.1 micrograms and necrosis at a higher dose (i.e., 5 micrograms). We conclude that the V. cholerae HA/protease and the P. aeruginosa elastase are structurally, functionally, and immunologically related.
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Authors | C C Häse, R A Finkelstein |
Journal | Infection and immunity
(Infect Immun)
Vol. 58
Issue 12
Pg. 4011-5
(Dec 1990)
ISSN: 0019-9567 [Print] United States |
PMID | 2123831
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Hemagglutinins
- Endopeptidases
- Pancreatic Elastase
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Topics |
- Amino Acid Sequence
- Animals
- Bacterial Adhesion
- Cross Reactions
- Endopeptidases
(analysis, immunology, toxicity)
- Hemagglutinins
(analysis, immunology, toxicity)
- Molecular Sequence Data
- Pancreatic Elastase
(analysis, immunology, toxicity)
- Pseudomonas aeruginosa
(enzymology)
- Rabbits
- Vibrio cholerae
(enzymology, pathogenicity)
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