Use of CD25 as an immunohistochemical marker for acquired ocular toxoplasmosis.

Abstract

PURPOSE:

Toxoplasmosis is the most common cause of posterior infectious uveitis worldwide. It is often impossible to determine its congenital or acquired nature. Interleukin-2 (IL-2) in peripheral blood has been described as a possible marker for acquired toxoplasmosis. The purpose of this study is to evaluate the histopathological characteristics of ocular toxoplasmosis cases using CD25 as a marker for the expression of interleukin-2.

METHODS:

Ten formalin-fixed, paraffin-embedded enucleated globes from ten immunocompetent patients with clinical diagnosis of toxoplasmosis were evaluated. Four patients had the acquired form of ocular toxoplasmosis (positive IgM) while six were IgM negative and IgG positive for toxoplasmosis. Histopathological slides were reviewed for the extension of the retinal necrosis, number of toxo cysts, the granulomatous inflammatory reaction, the presence of T and B cells within the choroid and the IL-2 expression. Immunohistochemistry using monoclonal antibodies was performed to observe the expression of CD4, CD8, CD20, CD25, and CD68.

RESULTS:

The histopathological evaluation disclosed no differences between acquired and the other ocular toxoplasmosis cases regarding the characteristics studied. However, CD25 showed a higher expression of IL-2 on the 4 acquired cases of ocular toxoplasmosis compared to the remainders.

CONCLUSIONS:

To the best of our knowledge, this is the first report showing that the use of CD25 as a marker for interleukin-2 could differentiate acquired ocular toxoplasmosis.

• Authors: Cristina Miyamoto, Rubens Belfort Mattos Neto, Sebastian Di Cesare, Rubens Belfort Junior, Miguel N Burnier Jr
• Journal: Arquivos brasileiros de oftalmologia (Arq Bras Oftalmol) 2010 Sep-Oct Vol. 73 Issue 5 Pg. 443-6 ISSN: 1678-2925 [Electronic] Brazil
• PMID: 21225130 (Publication Type: Journal Article)

Chemical References

• Biomarkers
• Immunoglobulin G
• Immunoglobulin M
• Interleukin-2
• Interleukin-2 Receptor alpha Subunit

Topics

• Biomarkers (analysis)
• Humans
• Immunoglobulin G (analysis)
• Immunoglobulin M (analysis)
• Interleukin-2 (analysis)
• Interleukin-2 Receptor alpha Subunit (immunology)
• Toxoplasmosis, Ocular (pathology)