Neoplastic alteration of a membrane-associated sialidase of rat liver.

Abstract

Rat liver particulate fraction contains two types of membrane-associated and gangliosides-hydrolyzing sialidase, which have been shown to be identical to two membrane-associated sialidases of rat brain (I and II) chromatographically, immunologically and in substrate specificity. Chromatography on AH-Sepharose 4B of the membrane sialidases of rat primary hepatoma induced by 3'-methyl-4-dimethylaminoazobenzene (MeDAB) further revealed that hepatocarcinogenesis induces a marked decrease in sialidase II but no decrease in sialidase I. Using antisera against sialidases I and II of rat brain, immunoprecipitation studies of the solubilized particulate fractions of rat liver and MeDAB-hepatoma gave results similar to those obtained chromatographically. Using the same immunological technique, sialidase II but not sialidase I was found to be decreased in AH109 A hepatoma and in regenerating and fetal liver.

• Authors: T Miyagi, K Konno, J Sagawa, S Tsuiki
• Journal: Japanese journal of cancer research : Gann (Jpn J Cancer Res) Vol. 81 Issue 9 Pg. 915-9 (Sep 1990) ISSN: 0910-5050 [Print] Japan
• PMID: 2121693 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Neuraminidase

Topics

• Animals
• Brain (enzymology)
• Cell Membrane (enzymology)
• Liver (enzymology)
• Liver Neoplasms (enzymology)
• Liver Neoplasms, Experimental (enzymology)
• Male
• Neuraminidase (metabolism)
• Precipitin Tests
• Rats
• Rats, Inbred Strains