Abstract |
In humans and other mammals, the α-chain of interleukin-2 (IL-2) receptor (CD25) is induced and expressed on the cell surface after lymphocyte activation and is released from the membrane of activated cells as a smaller soluble form (sCD25). However, little is known about avian sCD25. In the present study, we developed an antigen capture enzyme-linked immunosorbent assay (AC-ELISA) to detect serum sCD25 in ducks, and we used flow cytometry (FCM) to analyze the frequency of CD25(+) cells in the peripheral blood of ducks infected with H9N2 or H5N1 avian influenza virus (AIV) or serotype II Riemerella anatipestifer (RA). Using the AC-ELISA, duck sCD25 molecules were detected in the supernatant and lysates of concanavalin A (Con A)-stimulated peripheral blood mononuclear cells (PBMC), and in the serum of ducks infected with H5N1 virus and RA. However, no sCD25 was detected in the serum of H9N2 AIV-infected ducks. FCM analysis revealed that CD25(+) cells were upregulated within the PBMC of RA-infected ducks throughout the experiment until death, while in the PBMC of H9N2- and H5N1 AIV-infected ducks, the frequency of CD25(+) cells increased in the early stage of infection and then returned to a lower level. Our findings confirm that the dynamics of sCD25 and CD25(+) cells are different in the peripheral blood of ducks infected with H9N2 virus, H5N1 virus, and RA.
|
Authors | Zhenyu Huang, Jie Fang, Jianyou Gu, Yan Yan, Jiyong Zhou |
Journal | Veterinary immunology and immunopathology
(Vet Immunol Immunopathol)
Vol. 140
Issue 1-2
Pg. 102-9
(Mar 15 2011)
ISSN: 1873-2534 [Electronic] Netherlands |
PMID | 21216015
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright © 2010 Elsevier B.V. All rights reserved. |
Chemical References |
- Interleukin-2 Receptor alpha Subunit
- Concanavalin A
|
Topics |
- Animals
- Concanavalin A
(immunology)
- Ducks
(blood, immunology, microbiology, virology)
- Enzyme-Linked Immunosorbent Assay
(methods, veterinary)
- Flavobacteriaceae
(immunology)
- Flavobacteriaceae Infections
(immunology, veterinary)
- Flow Cytometry
(veterinary)
- Influenza A Virus, H5N1 Subtype
(immunology)
- Influenza A Virus, H9N2 Subtype
(immunology)
- Influenza in Birds
(immunology)
- Interleukin-2 Receptor alpha Subunit
(biosynthesis, blood)
- Kinetics
- Leukocytes, Mononuclear
(metabolism)
- Lymphocyte Count
(veterinary)
- T-Lymphocytes
(metabolism)
|