Abstract |
Several studies have highlighted the importance of murine natural killer (NK) cells in the control of influenza virus infection, notably through the natural cytotoxicity receptor NKp46. However, little is known about the involvement of NK cells in human influenza infection. Here, we show that upon in vitro exposure to influenza, NKp46 expression on NK cells decreases, whereas expression of 2B4, an activating receptor that can enhance natural cytotoxicity in synergy with NKp46, is up-regulated. Consistent with these observations, NKp46(dull) and 2B4(bright) NK cells had a higher functional activity in response to influenza than NK cells expressing high levels of NKp46 or low levels of 2B4, respectively. Importantly, we assessed whether the expression of these receptors was also modified in vivo in response to influenza antigens and showed that an increase in 2B4-expressing NK cells and a decrease in NKp46(+) NK cells occurred following intramuscular influenza vaccination. Altogether, our results further suggest that NKp46 may play an important role in the innate immune response to human influenza and reveal that exposure to influenza antigens is associated with a previously unrecognized increase in 2B4 expression that can impact NK cell activity against the virus.
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Authors | Stephanie Jost, Jeff Reardon, Eric Peterson, Danielle Poole, Ronald Bosch, Galit Alter, Marcus Altfeld |
Journal | Immunology
(Immunology)
Vol. 132
Issue 4
Pg. 516-26
(Apr 2011)
ISSN: 1365-2567 [Electronic] England |
PMID | 21214542
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2011 The Authors. Immunology © 2011 Blackwell Publishing Ltd. |
Chemical References |
- Antigens, CD
- CD244 protein, human
- Influenza Vaccines
- Lysosomal-Associated Membrane Protein 1
- NCR1 protein, human
- Natural Cytotoxicity Triggering Receptor 1
- Receptors, Immunologic
- Signaling Lymphocytic Activation Molecule Family
- Interferon-gamma
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Topics |
- Adult
- Antigens, CD
(immunology, metabolism)
- Cell Line, Tumor
- Cell Proliferation
- Cells, Cultured
- Female
- Flow Cytometry
- Host-Pathogen Interactions
(immunology)
- Humans
- Influenza A Virus, H1N1 Subtype
(immunology, physiology)
- Influenza Vaccines
(immunology)
- Interferon-gamma
(immunology, metabolism)
- K562 Cells
- Killer Cells, Natural
(immunology, metabolism, virology)
- Lysosomal-Associated Membrane Protein 1
(immunology, metabolism)
- Male
- Middle Aged
- Natural Cytotoxicity Triggering Receptor 1
(immunology, metabolism)
- Receptors, Immunologic
(immunology, metabolism)
- Signaling Lymphocytic Activation Molecule Family
- Vaccination
- Young Adult
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