Proline rich polypeptide (PRP-1) increases the superoxide-producing and ferrihemoglobin reducing activities of cytochrome B(558) isoforms from human lymphosarcoma tissue cells.

The two cytochromes (cyt) b(558) of acidic nature, one-95-100 kDa and another one, 60-70 kDa were isolated for the first time from the human's lymphosarcoma tissue cells using gel filtration and ion exchange chromatography. These hemoproteins possess NADPH dependent O(2)(-)-producing and ferrihemoglobin-reducing activities. The incubation of neuropeptide PRP-1 (5 μg) with cytochrome b(558), caused elevation of these activities. The gel filtration results indicated possible binding of PRP-1 to these cytochromes b(558). PRP-1 activated both NADPH dependent O(2)(-)-producing and ferriHb-reducing activities of the cyt b(1)(558) and cyt b(2)(558), obtained from human lymphosarcoma tissue cells. One can assume that PRP-1 associated with cyt b(558) on the surface of the tumor cells by increasing both NADPH dependent O(2)(-)-producing and ferriHb-reducing activities of cyt b(558), increases the oxidation- reduction status. Changing the oxidation-reduction status and oxygen homeostasis of the tumor cells by PRP-1 can serve as one of the possible explanation of antitumorigenic effect of this cytokine.
AuthorsG M Simonyan, K A Galoian, R M Simonyan, M A Simonyan, A A Galoyan
JournalNeurochemical research (Neurochem Res) Vol. 36 Issue 5 Pg. 739-45 (May 2011) ISSN: 1573-6903 [Electronic] United States
PMID21213045 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Cytochrome b Group
  • Isoenzymes
  • PRP-1 peptide
  • Peptides
  • Superoxides
  • Methemoglobin
  • cytochrome b558
  • NADPH Oxidase
  • Cytochrome b Group (metabolism)
  • Humans
  • Isoenzymes (metabolism)
  • Methemoglobin (metabolism)
  • NADPH Oxidase (metabolism)
  • Peptides (pharmacology)
  • Spectrum Analysis (methods)
  • Superoxides (metabolism)
  • Tumor Cells, Cultured

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: