Atrial natriuretic peptide alleviates cardiovascular and metabolic disorders in a rat endotoxemia model: a possible role for its anti-inflammatory properties.

Abstract

BACKGROUND:

Atrial natriuretic peptide (ANP) plays important roles in the regulation of cardiovascular and renal homeostasis. Furthermore, several studies have shown that ANP may have anti-inflammatory activities. We hypothesized that ANP may alleviate cardiovascular and/or metabolic disorders in rats with lipopolysaccharide (LPS)-induced endotoxemia.

METHODS:

In rats anesthetized with pentobarbital, LPS was injected and ANP was continuously infused at 0.15 µg/kg/min. Mean arterial pressure and pulse rate were monitored hourly, and arterial blood gases were analyzed before LPS injection and at 1, 4, and 6 hours after LPS injection. The expression in the rat left ventricle of mRNAs encoding nitric oxide synthase 2 and 3 (iNOS, eNOS), heme oxygenase 1 and 2 (HO-1, 2), tumor necrosis factor α (TNFα), and interleukin (IL)-1β was measured with the real-time reverse transcriptase-polymerase chain reaction.

RESULTS:

LPS increased the expression of TNFα, IL-1β, iNOS, and HO-1, which was inhibited by infusion of ANP. Furthermore, the LPS-induced decrease in mean arterial pressure was attenuated, and the acid-base imbalance caused by increased lactate production was improved 6 hours after the administration of ANP.

CONCLUSIONS:

Our results suggest that continuous infusion of ANP counteracts the cardiovascular and metabolic disorders associated with endotoxemia, possibly via anti-inflammatory mechanisms.

• Authors: Masaki Mori, Yoshitaka Yamanashi, Katsuya Kobayashi, Atsuhiro Sakamoto
• Journal: Journal of Nippon Medical School = Nippon Ika Daigaku zasshi (J Nippon Med Sch) Vol. 77 Issue 6 Pg. 296-305 (Dec 2010) ISSN: 1345-4676 [Print] Japan
• PMID: 21206142 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Lipopolysaccharides
• Atrial Natriuretic Factor

Topics

• Acidosis (drug therapy)
• Animals
• Anti-Inflammatory Agents (pharmacology)
• Atrial Natriuretic Factor (pharmacology, therapeutic use)
• Blood Pressure (drug effects)
• Cardiovascular Diseases (drug therapy)
• Endotoxemia (drug therapy)
• Lipopolysaccharides (toxicity)
• Male
• Rats
• Rats, Wistar