[Effect of nylestriol and levonorgestrel on the expression of estrogen receptor subtypes in human osteosarcoma MG-63 cell lines].

Abstract

OBJECTIVE:

To observe the effect of different concentrations of nylestriol (NYL) and levonorgestrel (LNG) on the expression of ERα and ERβ in human osteoscarcoma MG-63 cell lines, and to explore the impact of paracrine effect on the gene expression.

METHODS:

MG-63 cells were treated with 3 concentrations (10(-10),10(-8), and 10(-6) mol/L) of NYL or LNG. The untreated control group and the positive control group were also established. The 2 groups treated with NYL (10(-10) mol/L) or LNG (10(-8) mol/L) were designed to renew the medium every 12 h. Semi-quantitative RT-PCR was conducted to detect the mRNA expression of ERα and ERβ on the MG-63 cells treated with different concentrations of the 2 drugs, respectively.

RESULTS:

Both drugs up-regulated ERα and ERβ mRNA expression. The best concentration for both NYL and LNG was 10(-6) mol/L for ERα expression. As for ERβ, the best concentration of NYL and LNG was 10(-10) mol/L and 10(-8) mol/L. The role of medium replacement on the expression of ERα was not observed, but medium replacement inhibited ERβ expression.

CONCLUSION:

Both NYL and LNG can up-regulate the mRNA expression of ER subtypes in MG-63 cells, with mutual restriction between the 2 subtypes. The paracrine effect on MG-63 cell lines may be involved in the regulation process of mRNA expression of ERβ.

• Authors: Kaichu Yang, Eryuan Liao, Houde Zhou
• Journal: Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences (Zhong Nan Da Xue Xue Bao Yi Xue Ban) Vol. 35 Issue 12 Pg. 1248-53 (Dec 2010) ISSN: 1672-7347 [Print] China
• PMID: 21200092 (Publication Type: Journal Article)

Chemical References

• Estrogen Receptor alpha
• Estrogen Receptor beta
• RNA, Messenger
• Levonorgestrel
• nylestriol
• Quinestrol

Topics

• Cell Line, Tumor
• Estrogen Receptor alpha (genetics, metabolism)
• Estrogen Receptor beta (genetics, metabolism)
• Humans
• Levonorgestrel (pharmacology)
• Osteosarcoma (metabolism, pathology)
• Quinestrol (analogs & derivatives, pharmacology)
• RNA, Messenger (genetics, metabolism)