Abstract | BACKGROUND:
Heat shock protein 47 (HSP47) is a product of CBP2 gene located at chromosome 11q13.5, a region frequently amplified in human cancers. Areca quid chewing is a major risk factor of oral squamous cell carcinoma (OSCC). The aim of this study was to compare HSP47 expression in normal human oral epithelium and OSCC and further to explore the potential mechanisms that may lead to induce HSP47 expression. METHODS: RESULTS: HSP47 expression was significantly higher in OSCC specimens than normal epithelium (P<0.05). No significant difference in HSP47 expression was observed with respect to age, sex, T category, stage, and differentiation (P>0.05). The lower HSP47 expression was associated with lymph node metastasis (P=0.015). Arecoline was found to elevate HSP47 expression in a dose- and time-dependent manner (P<0.05). The addition of NAC, PD98059, LY294002, NS398, and herbimycin A markedly inhibited the arecoline-induced HSP47 expression (P<0.05). CONCLUSION:
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Authors | Shiuan-Shinn Lee, Ling-Hsien Tseng, Yi-Ching Li, Chung-Hung Tsai, Yu-Chao Chang |
Journal | Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
(J Oral Pathol Med)
Vol. 40
Issue 5
Pg. 390-6
(May 2011)
ISSN: 1600-0714 [Electronic] Denmark |
PMID | 21198874
(Publication Type: Journal Article)
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Copyright | © 2010 John Wiley & Sons A/S. |
Chemical References |
- Cholinergic Agonists
- HSP47 Heat-Shock Proteins
- Arecoline
- Cyclooxygenase 2
- Phosphatidylinositol 3-Kinase
- Protein-Tyrosine Kinases
- Extracellular Signal-Regulated MAP Kinases
- Glutathione
- Acetylcysteine
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Topics |
- Acetylcysteine
(pharmacology)
- Areca
(adverse effects)
- Arecoline
(antagonists & inhibitors, pharmacology)
- Carcinoma, Squamous Cell
(chemically induced, metabolism, pathology)
- Case-Control Studies
- Cell Line, Tumor
- Cholinergic Agonists
(pharmacology)
- Cyclooxygenase 2
(pharmacology)
- Epithelial Cells
(drug effects, metabolism)
- Extracellular Signal-Regulated MAP Kinases
(pharmacology)
- Female
- Gene Expression Regulation, Neoplastic
(drug effects)
- Glutathione
(pharmacology)
- HSP47 Heat-Shock Proteins
(biosynthesis)
- Humans
- Lymphatic Metastasis
- Male
- Middle Aged
- Mouth Mucosa
(cytology, drug effects, metabolism)
- Mouth Neoplasms
(chemically induced, metabolism, pathology)
- Neoplasm Staging
- Phosphatidylinositol 3-Kinase
(pharmacology)
- Protein-Tyrosine Kinases
(pharmacology)
- Signal Transduction
- Up-Regulation
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