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Therapeutic effect of photodynamic therapy using Na-pheophorbide a on osteomyelitis models in rats.

AbstractOBJECTIVE:
In this study, we examined the therapeutic effect of photodynamic therapy (PDT) using the photosensitizer Na-Pheophorbide a (Na-Phde a) on osteomyelitis models in rats.
BACKGROUND:
Osteomyelitis is one of the most serious infectious problems in the orthopedic field. Recently, as a new clinical approach against septic arthritis, an experimental in vivo and in vitro model for the inactivation of methicillin-resistant-Staphylococcus aureus by PDT using Na-Phde a has been developed.
METHODS:
Methicillin-sensitive Staphylococcus aureus (MSSA) was injected into the tibia of the rats to create osteomyelitis models (n = 10, 10 legs). A total of 560 μmol/l of Na-Phde a solution was injected into five of these tibial osteomyelitis models (five legs) 48 h after the initial MSSA infection. Sixty minutes after the Na-Phde a injection, a semiconductor laser (125 mW, 670 nm) was used to irradiate the models for 10 min with a total energy of 93.8 J/mm(2). As a control group, five rats (five legs) were treated with a phosphate buffered saline injection at 48 h after MSSA infection. Weight and leg perimeter changes were plotted. Bacterial growth, histological examination and radiological examination were evaluated at 14 days after initial treatment.
RESULTS:
PDT with Na-Phde a significantly prevented leg swelling. In the PDT group, bone destruction owing to osteomyelitis was inhibited not only histologically but also radiographically.
CONCLUSIONS:
The results in these experiments show that PDT using Na-Phde a improved osteomyelitis in rats. This suggests that PDT using Na- Phde a can be a useful treatment for osteomyelitis.
AuthorsBunsei Goto, Takanori Iriuchishima, Takashi Horaguchi, Yasuaki Tokuhashi, Yu Nagai, Tomonori Harada, Akiyoshi Saito, Shin Aizawa
JournalPhotomedicine and laser surgery (Photomed Laser Surg) Vol. 29 Issue 3 Pg. 183-9 (Mar 2011) ISSN: 1557-8550 [Electronic] United States
PMID21194297 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Photosensitizing Agents
  • Chlorophyll
  • pheophorbide a
Topics
  • Animals
  • Biopsy, Needle
  • Chlorophyll (analogs & derivatives, pharmacology)
  • Disease Models, Animal
  • Female
  • Immunohistochemistry
  • Osteomyelitis (drug therapy, microbiology)
  • Photochemotherapy (methods)
  • Photosensitizing Agents (pharmacology)
  • Random Allocation
  • Rats
  • Rats, Sprague-Dawley
  • Reference Values
  • Staphylococcal Infections (drug therapy)
  • Statistics, Nonparametric
  • Tibia (drug effects, microbiology)
  • Treatment Outcome

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