Abstract | OBJECTIVE: BACKGROUND:
Osteomyelitis is one of the most serious infectious problems in the orthopedic field. Recently, as a new clinical approach against septic arthritis, an experimental in vivo and in vitro model for the inactivation of methicillin-resistant-Staphylococcus aureus by PDT using Na-Phde a has been developed. METHODS:
Methicillin-sensitive Staphylococcus aureus (MSSA) was injected into the tibia of the rats to create osteomyelitis models (n = 10, 10 legs). A total of 560 μmol/l of Na-Phde a solution was injected into five of these tibial osteomyelitis models (five legs) 48 h after the initial MSSA infection. Sixty minutes after the Na-Phde a injection, a semiconductor laser (125 mW, 670 nm) was used to irradiate the models for 10 min with a total energy of 93.8 J/mm(2). As a control group, five rats (five legs) were treated with a phosphate buffered saline injection at 48 h after MSSA infection. Weight and leg perimeter changes were plotted. Bacterial growth, histological examination and radiological examination were evaluated at 14 days after initial treatment. RESULTS:
PDT with Na-Phde a significantly prevented leg swelling. In the PDT group, bone destruction owing to osteomyelitis was inhibited not only histologically but also radiographically. CONCLUSIONS: The results in these experiments show that PDT using Na-Phde a improved osteomyelitis in rats. This suggests that PDT using Na- Phde a can be a useful treatment for osteomyelitis.
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Authors | Bunsei Goto, Takanori Iriuchishima, Takashi Horaguchi, Yasuaki Tokuhashi, Yu Nagai, Tomonori Harada, Akiyoshi Saito, Shin Aizawa |
Journal | Photomedicine and laser surgery
(Photomed Laser Surg)
Vol. 29
Issue 3
Pg. 183-9
(Mar 2011)
ISSN: 1557-8550 [Electronic] United States |
PMID | 21194297
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Photosensitizing Agents
- Chlorophyll
- pheophorbide a
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Topics |
- Animals
- Biopsy, Needle
- Chlorophyll
(analogs & derivatives, pharmacology)
- Disease Models, Animal
- Female
- Immunohistochemistry
- Osteomyelitis
(drug therapy, microbiology)
- Photochemotherapy
(methods)
- Photosensitizing Agents
(pharmacology)
- Random Allocation
- Rats
- Rats, Sprague-Dawley
- Reference Values
- Staphylococcal Infections
(drug therapy)
- Statistics, Nonparametric
- Tibia
(drug effects, microbiology)
- Treatment Outcome
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