HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Avian CD154 enhances humoral and cellular immune responses induced by an adenovirus vector-based vaccine in chickens.

Abstract
Recombinant adenoviral vectors have emerged as an attractive system for veterinary vaccines development. However, for poultry vaccination a very important criterion for an ideal vaccine is its low cost. The objective of this study was to test the ability of chicken CD154 to enhance the immunogenicity of an adenoviral vector-based vaccine against avian influenza virus in order to reduce the amount of antigen required to induce an effective immune response in avian. Chickens were vaccinated with three different doses of adenoviral vectors encoding either HA (AdHA), or HA fused to extracellular domain chicken's CD154 (AdHACD). Hemagglutination inhibition (HI) assay and relative quantification of IFN-γ showed that the adenoviral vector encoding for the chimeric antigen is able to elicit an improved humoral and cellular immune response, which demonstrated that CD154 can be used as a molecular adjuvant allowing to reduce in about 50-fold the amount of adenoviral vector vaccine required to induce an effective immune response.
AuthorsOliberto Sánchez Ramos, Alain González Pose, Silvia Gómez-Puerta, Julia Noda Gomez, Armando Vega Redondo, Julio César Águila Benites, Lester Suárez Amarán, Natalie C Parra, Jorge R Toledo Alonso
JournalComparative immunology, microbiology and infectious diseases (Comp Immunol Microbiol Infect Dis) Vol. 34 Issue 3 Pg. 259-65 (May 2011) ISSN: 1878-1667 [Electronic] England
PMID21190734 (Publication Type: Journal Article)
CopyrightCopyright © 2010 Elsevier Ltd. All rights reserved.
Chemical References
  • Antibodies, Viral
  • Recombinant Fusion Proteins
  • Viral Vaccines
  • CD40 Ligand
  • Interferon-gamma
Topics
  • Adenoviridae (genetics)
  • Animals
  • Antibodies, Viral (blood)
  • CD40 Ligand (genetics, immunology)
  • Cell Line
  • Chickens
  • Gene Order
  • Genetic Vectors (genetics)
  • Immunity, Cellular (immunology)
  • Immunity, Humoral (immunology)
  • Interferon-gamma (immunology)
  • Leukocytes, Mononuclear (immunology)
  • Mice
  • Recombinant Fusion Proteins (genetics, immunology)
  • Viral Vaccines (genetics, immunology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: