Abstract | AIM: To investigate protective effects of polydatin(PD) during lung ischemia/reperfusion in rabbits and its potential mechanisms. METHODS: Rabbit lung model of ischemia/reperfusion (I/R) injury was constituted in vivo. Thirty rabbits were divided into groups randomly: Control (C), I/R, PD group, respectively. Endotoxin (ET) in plasma was analyzed by End-point Chromogenic Assay, the expression of Toll-like receptor 4 (TLR4) mRNA, nuclear factor (NF)-kappaBp65 mRNA, intracellular adhesion molecule-1 (ICAM-1) mRNA were measured by RT-PCR, the morphological changes of lung tissue were observed with hematoxylin- eosin (HE) staining. RESULTS: There was no significant difference in ET concentration of plasma between groups (all of P > 0.05). The expression of TLR-4 mRNA, NF-kappaBp65 mRNA and ICAM-1mRNA in I/R group were significantly increased as compared to C group and PD group, while those expressions in PD group were evidently higher than those in C group (all of P < 0.01). Light microscope showed that the lung pathological injuries in PD group were obviously alleviated as compared to I/R group. CONCLUSION:
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Authors | Xiao-Feng Jin, Zheng-Jie Xu, Wan-Tie Wang, Yi-Xiao Xu, Xiao-Long Zhang |
Journal | Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology
(Zhongguo Ying Yong Sheng Li Xue Za Zhi)
Vol. 25
Issue 1
Pg. 41-4
(Feb 2009)
ISSN: 1000-6834 [Print] China |
PMID | 21186612
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Glucosides
- Protective Agents
- RNA, Messenger
- Stilbenes
- Toll-Like Receptor 4
- Transcription Factor RelA
- Intercellular Adhesion Molecule-1
- polydatin
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Topics |
- Animals
- Female
- Glucosides
(pharmacology)
- Intercellular Adhesion Molecule-1
(genetics, metabolism)
- Ischemia
(metabolism, physiopathology)
- Lung
(blood supply)
- Male
- Protective Agents
(pharmacology)
- RNA, Messenger
(genetics, metabolism)
- Rabbits
- Reperfusion Injury
(metabolism, prevention & control)
- Signal Transduction
(drug effects)
- Stilbenes
(pharmacology)
- Toll-Like Receptor 4
(genetics, metabolism)
- Transcription Factor RelA
(genetics, metabolism)
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