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[The regulative effects of polydatin on toll-like receptor 4 signal transduction pathway in lung ischemia/reperfusion injury in rabbits].

AbstractAIM:
To investigate protective effects of polydatin(PD) during lung ischemia/reperfusion in rabbits and its potential mechanisms.
METHODS:
Rabbit lung model of ischemia/reperfusion (I/R) injury was constituted in vivo. Thirty rabbits were divided into groups randomly: Control (C), I/R, PD group, respectively. Endotoxin (ET) in plasma was analyzed by End-point Chromogenic Assay, the expression of Toll-like receptor 4 (TLR4) mRNA, nuclear factor (NF)-kappaBp65 mRNA, intracellular adhesion molecule-1 (ICAM-1) mRNA were measured by RT-PCR, the morphological changes of lung tissue were observed with hematoxylin-eosin (HE) staining.
RESULTS:
There was no significant difference in ET concentration of plasma between groups (all of P > 0.05). The expression of TLR-4 mRNA, NF-kappaBp65 mRNA and ICAM-1mRNA in I/R group were significantly increased as compared to C group and PD group, while those expressions in PD group were evidently higher than those in C group (all of P < 0.01). Light microscope showed that the lung pathological injuries in PD group were obviously alleviated as compared to I/R group.
CONCLUSION:
PD might have a protective effect on lung ischemia/reperfusion injury by down-regulating TLR4 and NF-kappaB expression, then inhibiting the release of mediators of inflammation as ICAM-1.
AuthorsXiao-Feng Jin, Zheng-Jie Xu, Wan-Tie Wang, Yi-Xiao Xu, Xiao-Long Zhang
JournalZhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology (Zhongguo Ying Yong Sheng Li Xue Za Zhi) Vol. 25 Issue 1 Pg. 41-4 (Feb 2009) ISSN: 1000-6834 [Print] China
PMID21186612 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Glucosides
  • Protective Agents
  • RNA, Messenger
  • Stilbenes
  • Toll-Like Receptor 4
  • Transcription Factor RelA
  • Intercellular Adhesion Molecule-1
  • polydatin
Topics
  • Animals
  • Female
  • Glucosides (pharmacology)
  • Intercellular Adhesion Molecule-1 (genetics, metabolism)
  • Ischemia (metabolism, physiopathology)
  • Lung (blood supply)
  • Male
  • Protective Agents (pharmacology)
  • RNA, Messenger (genetics, metabolism)
  • Rabbits
  • Reperfusion Injury (metabolism, prevention & control)
  • Signal Transduction (drug effects)
  • Stilbenes (pharmacology)
  • Toll-Like Receptor 4 (genetics, metabolism)
  • Transcription Factor RelA (genetics, metabolism)

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