[Expression and function analysis of dengue virus type 1 to 4 envelope domain III recombinant fusion protein].

Abstract

OBJECTIVE:

To observe the ability of dengue virus type 1-4 envelope domain III fusion protein to inhibit virus infection and analyze the neutralizing ability of polyclonal antibodies against rE III.

METHODS:

After being connected by linker peptide, E III protein of Dengue virus serotypes 1-4 were expressed in E coli BL21 (DE3) then purified. Fusion proteins were verified by Western Blot and ELISA. Rabbits were immunized with fusion proteins to produce anti-rE III serum. The activity of anti-rE III serum were detected through indirect immunofluorescence assay test. Inhibition of dengue virus type 1 to 4 infection in BHK-21 cells by rE III fusion protein were tested. Neutralizing activity of anti-rE III serum was analyzed.

RESULTS:

Dengue virus type 1 to 4 envelope domain III recombinant fusion protein was expressed in E coli BL21 and purified successfully. Then rE III fusion protein and anti-rE III serum were analyzed respectively and rE III fusion protein can effectively inhibit dengue virus type 1 to 4 from infecting BHK cells. The anti-rE III serums can neutralize dengue virus type 1 to 4 but with different neutralizing titer.

CONCLUSION:

Dengue virus type 1-4 envelope domain III fusion protein can directly inhibit DV infection. Antibodies induced by rE III fusion proteins can neutralize dengue virus type 1-4.

• Authors: Guo-Yu Niu, Peng Lu, Shuo Zhang, Quan-Fu Zhang, Chuan Li, Mi-Fang Liang, Fang Xu, De-Xin Li
• Journal: Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology (Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi) Vol. 24 Issue 3 Pg. 161-4 (Jun 2010) ISSN: 1003-9279 [Print] China
• PMID: 21186514 (Publication Type: Journal Article)

Chemical References

• Gene Products, env
• Recombinant Fusion Proteins
• Recombinant Proteins
• Viral Envelope Proteins

Topics

• Animals
• Blotting, Western
• Cells, Cultured
• Dengue Virus (classification, drug effects, growth & development)
• Enzyme-Linked Immunosorbent Assay
• Escherichia coli (genetics)
• Gene Fusion (genetics)
• Gene Products, env (genetics, metabolism)
• Immunization
• Rabbits
• Recombinant Fusion Proteins (genetics, immunology, metabolism, pharmacology)
• Recombinant Proteins (biosynthesis, pharmacology)
• Viral Envelope Proteins (genetics, immunology, metabolism)
• Virus Replication (drug effects, physiology)