Determination of firocoxib in equine plasma using high performance liquid chromatography.

Abstract

A new method of analysis has been developed and validated for the determination of firocoxib, a new nonsteroidal anti-inflammatory drug (NSAID) approved for use in horses and dogs to control pain and inflammation associated with osteoarthritis. Following a liquid extraction using ethyl acetate:hexane (40:60), samples were separated by isocratic reversed-phase HPLC on a Sunfire C(18) column and quantified using UV detection at 290 nm. The mobile phase was a mixture of water with 0.025% trifluoroacetic acid and acetonitrile, with a flow-rate of 1.1 ml/min. The procedure produced a linear curve over the concentration range 5-1500 ng/ml with a lower limit of quantification of 5 ng/ml. Intra- and inter-assay variability was less than 7%. The average recovery was 98%. The method is suitable for the analysis of clinical samples from pharmacokinetic studies and can also be used for small volume sample sizes.

• Authors: S Cox, J Yarbrough
• Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (J Chromatogr B Analyt Technol Biomed Life Sci) Vol. 879 Issue 2 Pg. 205-8 (Jan 15 2011) ISSN: 1873-376X [Electronic] Netherlands
• PMID: 21185237 (Publication Type: Journal Article)
• Copyright: Copyright © 2010 Elsevier B.V. All rights reserved.

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Sulfonamides
• Sulfones
• 4-Butyrolactone
• deracoxib
• firocoxib

Topics

• 4-Butyrolactone (analogs & derivatives, blood)
• Animals
• Anti-Inflammatory Agents, Non-Steroidal (blood)
• Chromatography, High Pressure Liquid (methods)
• Drug Stability
• Horses (blood)
• Linear Models
• Reproducibility of Results
• Sensitivity and Specificity
• Sulfonamides (analysis)
• Sulfones (blood)