Etiological role of human papillomavirus infection for inverted papilloma of the bladder.

Abstract

The status of human papillomavirus (HPV) infection in urothelial inverted papilloma was examined in the present study. Formalin-fixed and paraffin-embedded tissues from eight cases of inverted papilloma of the bladder were studied. The presence of HPV-DNA was examined by modified GP5/6+PCR using archival tissue sections by microdissection. HPV genotype was determined with a Hybri-Max HPV genotyping kit. Immunohistochemical analysis for p16-INK4a, mcm7, HPV-E4, and L1, and in situ hybridization for the HPV genome were performed. HPV was detected in seven of eight cases (87.5%) of inverted papilloma. Three cases were diagnosed as inverted papilloma with atypia, while the remaining five were typical cases. HPV-18 was detected in two cases, including one inverted papilloma with atypia, and HPV-16 was detected in four cases, including one inverted papilloma with atypia. Multiple HPV type infection was detected in one typical case and one atypical case. High-risk HPV was present in all HPV-positive cases. Cellular proteins, p16-INK4a and mcm7, which are surrogate markers for HPV-E7 expression, were detected in all HPV-positive cases, and their levels were higher in inverted papilloma with atypia than in typical cases. In contrast, HPV-E4 and L1, which are markers for HPV propagation, were observed in some parts of the typical inverted papilloma tissue. High-risk HPV infection may be one of the causes of urothelial inverted papilloma, and inverted papilloma with atypia may have malignant potential.

• Authors: Kazuyoshi Shigehara, Toshiyuki Sasagawa, John Doorbar, Shohei Kawaguchi, Yoshitomo Kobori, Takao Nakashima, Masayoshi Shimamura, Yuji Maeda, Tohru Miyagi, Yasuhide Kitagawa, Yoshifumi Kadono, Hiroyuki Konaka, Atsushi Mizokami, Eitetsu Koh, Mikio Namiki
• Journal: Journal of medical virology (J Med Virol) Vol. 83 Issue 2 Pg. 277-85 (Feb 2011) ISSN: 1096-9071 [Electronic] United States
• PMID: 21181923 (Publication Type: Case Reports, Journal Article)
• Copyright: 2010 Wiley-Liss, Inc.

Chemical References

• Capsid Proteins
• Cell Cycle Proteins
• Cyclin-Dependent Kinase Inhibitor p16
• DNA, Viral
• DNA-Binding Proteins
• HPV L1 protein, Human papillomavirus
• Nuclear Proteins
• Oncogene Proteins, Viral
• MCM7 protein, human
• Minichromosome Maintenance Complex Component 7

Topics

• Adult
• Aged
• Capsid Proteins (metabolism)
• Cell Cycle Proteins (metabolism)
• Cyclin-Dependent Kinase Inhibitor p16 (metabolism)
• DNA Replication
• DNA, Viral (analysis)
• DNA-Binding Proteins (metabolism)
• Female
• Human papillomavirus 16 (isolation & purification)
• Human papillomavirus 18 (isolation & purification)
• Humans
• In Situ Hybridization
• Male
• Middle Aged
• Minichromosome Maintenance Complex Component 7
• Nuclear Proteins (metabolism)
• Oncogene Proteins, Viral (metabolism)
• Papilloma, Inverted (virology)
• Papillomavirus Infections (complications, diagnosis)
• Polymerase Chain Reaction
• Urinary Bladder Neoplasms (virology)