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Assessment of Dietzia subsp. C79793-74 for treatment of cattle with evidence of paratuberculosis.

Abstract
The objective of the present investigation was to determine whether the bacterium Dietzia subsp. C79793-74, previously shown to inhibit growth of Mycobacterium subsp. paratuberculosis under in vitro culture conditions, has therapeutic value as a probiotic for adult cattle with paratuberculosis.  Animals were obtained from several herds with evidence of disease based on seropositivity and/or fecal shedding.  Sixty-eight cows with initial evidence of Stage II or III paratuberculosis and 2 with an initial Stage IV disease were evaluated longitudinally.  Animals were either treated daily with variable, disease-dependent doses of Dietzia (n = 48) or left untreated (n = 22).  Clinical aspects of disease (diarrhea, emaciated, cachectic and appetite) were recorded until the animal recovered or required euthanasia due to advanced clinical paratuberculosis or other severe conditions.  Paratuberculosis parameters-antibody serology (ELISA, AGID) and fecal culture-were longitudinally monitored over the lifetime of each animal.  The results indicated that daily treatment with Dietzia was therapeutic for paratuberculosis cows based on: (a) longitudinal decline in ELISA values only occurred in animals that were treated; (b) prolonged survival was dependant upon treatment--the length being directly associated with low initial ELISA values; and (c) treated animals were the only ones cured of disease.  Further investigations are envisaged to determine optimal, long-term dosages that may result in even better therapeutic outcomes as well as to evaluate potential application for therapy of the Johne's disease, human-counterpart, Crohn's disease.
AuthorsRobert E Click, Craig L Van Kampen
JournalVirulence (Virulence) 2010 May-Jun Vol. 1 Issue 3 Pg. 145-55 ISSN: 2150-5608 [Electronic] United States
PMID21178433 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies, Bacterial
Topics
  • Actinomycetales (immunology)
  • Animals
  • Antibodies, Bacterial (blood)
  • Cattle
  • Cattle Diseases (immunology, microbiology, physiopathology, therapy)
  • Colony Count, Microbial (methods, veterinary)
  • Enzyme-Linked Immunosorbent Assay
  • Feces (microbiology)
  • Mycobacterium avium subsp. paratuberculosis (growth & development, immunology, isolation & purification)
  • Paratuberculosis (immunology, microbiology, physiopathology, therapy)
  • Probiotics (administration & dosage, therapeutic use)
  • Treatment Outcome

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