Abstract |
The objective of the present investigation was to determine whether the bacterium Dietzia subsp. C79793-74, previously shown to inhibit growth of Mycobacterium subsp. paratuberculosis under in vitro culture conditions, has therapeutic value as a probiotic for adult cattle with paratuberculosis. Animals were obtained from several herds with evidence of disease based on seropositivity and/or fecal shedding. Sixty-eight cows with initial evidence of Stage II or III paratuberculosis and 2 with an initial Stage IV disease were evaluated longitudinally. Animals were either treated daily with variable, disease-dependent doses of Dietzia (n = 48) or left untreated (n = 22). Clinical aspects of disease ( diarrhea, emaciated, cachectic and appetite) were recorded until the animal recovered or required euthanasia due to advanced clinical paratuberculosis or other severe conditions. Paratuberculosis parameters-antibody serology (ELISA, AGID) and fecal culture-were longitudinally monitored over the lifetime of each animal. The results indicated that daily treatment with Dietzia was therapeutic for paratuberculosis cows based on: (a) longitudinal decline in ELISA values only occurred in animals that were treated; (b) prolonged survival was dependant upon treatment--the length being directly associated with low initial ELISA values; and (c) treated animals were the only ones cured of disease. Further investigations are envisaged to determine optimal, long-term dosages that may result in even better therapeutic outcomes as well as to evaluate potential application for therapy of the Johne's disease, human-counterpart, Crohn's disease.
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Authors | Robert E Click, Craig L Van Kampen |
Journal | Virulence
(Virulence)
2010 May-Jun
Vol. 1
Issue 3
Pg. 145-55
ISSN: 2150-5608 [Electronic] United States |
PMID | 21178433
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Actinomycetales
(immunology)
- Animals
- Antibodies, Bacterial
(blood)
- Cattle
- Cattle Diseases
(immunology, microbiology, physiopathology, therapy)
- Colony Count, Microbial
(methods, veterinary)
- Enzyme-Linked Immunosorbent Assay
- Feces
(microbiology)
- Mycobacterium avium subsp. paratuberculosis
(growth & development, immunology, isolation & purification)
- Paratuberculosis
(immunology, microbiology, physiopathology, therapy)
- Probiotics
(administration & dosage, therapeutic use)
- Treatment Outcome
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