Abstract | AIMS:
Thymidine kinase 1 (TK1) is an enzyme involved in DNA synthesis and an important proliferation marker. We explored the association of preoperative serum TK1 activity with clinicopathological parameters and prognosis in terms of recurrence-free survival (RFS) in breast cancer (BC) patients. PATIENTS AND METHODS: TK1 activity in serum of 120 healthy women and 161 BC patients was measured by quantitative ELISA. RESULTS: Serum TK1 activity in BC patients was significantly higher than in healthy women (P < 0.0001). In BC patients elevated TK1 activity was significantly associated with advanced T stage (P = 0.015), higher grade (P = 0.013), presence of tumor necrosis (P = 0.006), vascular invasion (P = 0.002), and lack of estrogen receptor (ER) and progesterone receptor (PR) expression (P = 0.0004 and P = 0.003). Higher TK1 activity was found in patients with BRCA1/2 mutations compared to those without the mutation (P = 0.004). Multivariate Cox proportional hazards analyses demonstrated that TK1, adjusted for stage, grade, necrosis, ER and PR negativity was retained as an independent predictor of disease recurrence (Hazard Ratio = 3.9, 95%CI 1.3-11.6, P = 0.013). CONCLUSION: Elevated serum TK1 is an important risk factor indicating a high proliferation potential of tumors at the time of excision. In multivariate analysis TK1 activity was found to be an independent prognostic factor for RFS.
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Authors | Benjamin Nisman, Tanir Allweis, Luna Kaduri, Bella Maly, Simon Gronowitz, Tamar Hamburger, Tamar Peretz |
Journal | Cancer biomarkers : section A of Disease markers
(Cancer Biomark)
Vol. 7
Issue 2
Pg. 65-72
( 2010)
ISSN: 1875-8592 [Electronic] Netherlands |
PMID | 21178264
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Biomarkers, Tumor
- Receptors, Estrogen
- Receptors, Progesterone
- Thymidine Kinase
- thymidine kinase 1
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Topics |
- Adult
- Aged
- Biomarkers, Tumor
(blood)
- Breast Neoplasms
(blood, enzymology, pathology)
- Case-Control Studies
- Disease-Free Survival
- Female
- Humans
- Middle Aged
- Neoplasm Recurrence, Local
(pathology)
- Neoplasm Staging
- Predictive Value of Tests
- Prognosis
- Prospective Studies
- Receptors, Estrogen
(metabolism)
- Receptors, Progesterone
(metabolism)
- Risk Factors
- Thymidine Kinase
(blood)
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