[Study on the relationship of metallothionein and antioxidases in protective mechanism of cardiomyocytes].

Abstract

AIM:

To study the relationship of Metallothionein (MT) and antioxidases in hypoxia/reoxygenation (H/R) and hypoxic preconditioning (HPC).

METHODS:

H/R and HPC model of rat cultured cardiomyocytes were established. Then the contents of MT and relevant changes of activities of antioxidases (SOD, CAT, GSHpx) of cardiomyocytes were determined while pretreated with the treatment: HPC. Zn2+ inducing MT or MT antibody.

RESULTS:

In HPC and Zn2+ induced groups, all the contents of MT and the activities of antioxidases were obviously higher than those in H/R (P < 0.05, P < 0.01 respectively). While using MT, the activities of SOD were progressively increased than control (P < 0.05), CAT and GSHpx activities were lower than control (P < 0.01), but were significantly higher than those in I/R group (P < 0.01). In MT antibody groups, the activities of antioxidase were decreased extremely.

CONCLUSION:

MT has effects on the cardiomyocytes protective role of HPC. And can afford more capacity for cardiomyocytes to tolerate the H/R damage by the mechanism of increasing the activities of antioxidase.

• Authors: H Y Jin, F Q Li, D X Fang, S Q Li, W G Tan, H B Chen
• Journal: Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology (Zhongguo Ying Yong Sheng Li Xue Za Zhi) Vol. 17 Issue 1 Pg. 61-3 (Feb 2001) ISSN: 1000-6834 [Print] China
• PMID: 21171446 (Publication Type: English Abstract, Journal Article)

Chemical References

• Metallothionein
• Catalase
• Glutathione Peroxidase
• Superoxide Dismutase

Topics

• Animals
• Catalase (metabolism)
• Cell Hypoxia
• Cells, Cultured
• Glutathione Peroxidase (metabolism)
• Ischemic Preconditioning
• Metallothionein (metabolism)
• Myocytes, Cardiac (metabolism)
• Rats
• Rats, Sprague-Dawley
• Superoxide Dismutase (metabolism)