[Effects of cyclooxygenase-1 inhibitor on spinal ERK expression in rats with postoperative pain].

Abstract

AIM:

To explore the effects of intrathecal injection of selective cyclooxygenase-1 (COX-1) inhibitor, SC-560, on mechanical allo dynia and spinal ERK protein expression in rats with postoperative pain.

METHODS:

Rats were divided into 4 groups: control group, postoperative pain group, SC-560 group and DMSO group. Mechanical withdrawal threshold (MWT), immunohistochemical and Western blotting technique were used to evaluate mechanical hypersensitivity and the expression of phospho-ERK in the spinal cord, respectively.

RESULTS:

(1) Behavior test rats developed allodynia 1 h after operation and SC-560 100 microg administrated intrathecally demonstrated a significant reduction in postoperative hypersensitivity. (2) Immunohistochemical staining Phospho-ERK positive neurons in the rat superficial spinal dorsal horn increased significantly 1 h after incision compared with that of non-incision group. Intrathecal administration of SC-560 preoperatively could significantly reduce the number of phospho-ERK positive neurons. (3) Western blot expression of phospho-ERK1/2 protein in the lumbar spinal cord increased significantly 1 h after incision and decreased by intrathecal injection of SC-560.

CONCLUSION:

SC-560 administrated intrathecally can inhibit mechanical hypersensitivity induced by postoperative pain in rats and this anti-allodynic process may mediated by spinal ERK.

• Authors: Yue-Tao Ma, Jun-Ke Wang
• Journal: Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology (Zhongguo Ying Yong Sheng Li Xue Za Zhi) Vol. 23 Issue 1 Pg. 46-50 (Feb 2007) ISSN: 1000-6834 [Print] China
• PMID: 21171365 (Publication Type: English Abstract, Journal Article)

Chemical References

• Cyclooxygenase Inhibitors
• Pyrazoles
• SC 560
• Extracellular Signal-Regulated MAP Kinases

Topics

• Animals
• Cyclooxygenase Inhibitors (pharmacology)
• Extracellular Signal-Regulated MAP Kinases (metabolism)
• Male
• Pain Measurement (drug effects)
• Pain, Postoperative (metabolism)
• Pyrazoles (pharmacology)
• Rats
• Rats, Wistar
• Spinal Cord (drug effects, metabolism)