[Effect of inhibiting endoxin by antidigoxin antiserum on myocardial ischemia/reperfusion injury in rats].

Abstract

AIM:

To study the effect of antidigoxin antiserum on oxygen stress induced by myocardial ischemia/reperfusion (MI/R) injury in rats.

METHODS:

Sprauge Dawley rats were submitted to ligate left anterior descending coronary artery 30 min followed by 45 min reperfusion. Experiment animals were randomly divided into seven groups including sham group, MI/R group, normal salina group, verapamil group and three antidigoxin antiserum groups from low to high dose. The left ventricular myocardial tissue sample of ischemia were processed and measured the level of endoxin and malondialdehyde (MDA), the activities of Na+, K(+) -ATPase and superoxin dismutase (SOD). The myocardia morphology was observed.

RESULTS:

The levels of endoxin and MDA increased and the activities of Na+, K(+) -ATPase and MDA were inhibited significantly in MI/R and saline groups. Including verapamil group in comparison to MI/R and saline groups, MDA level decreased and SOD activities partly reserved, meanwhile, only in three antidigoxin antiserum groups, the myocardial endoxin level was remarkably decreased, Na+, K(+) -ATPase activities were drastically increased. The myocardial histological morphology was significantly improved.

CONCLUSION:

Antidigoxin antiserum, an endoxin mutual clone antibody, had the effect of attenuating the damage of oxygen free radicals induced by MI/R via to antagonizing the inhibition effect of endoxin on myocardial membrane Na+, K(+) -ATPase activities.

• Authors: De-Guo Wang, He-Gui Wang, Yong-Sheng Ke, Shang-Yin Yang
• Journal: Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology (Zhongguo Ying Yong Sheng Li Xue Za Zhi) Vol. 23 Issue 1 Pg. 9-12 (Feb 2007) ISSN: 1000-6834 [Print] China
• PMID: 21171356 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cardenolides
• Immune Sera
• Saponins
• digoxin-like factors
• Malondialdehyde
• Digoxin
• Superoxide Dismutase
• Sodium-Potassium-Exchanging ATPase

Topics

• Animals
• Cardenolides (antagonists & inhibitors)
• Digoxin (pharmacology)
• Immune Sera (pharmacology)
• Malondialdehyde (analysis)
• Myocardial Reperfusion
• Myocardial Reperfusion Injury (physiopathology)
• Rats
• Rats, Sprague-Dawley
• Saponins (antagonists & inhibitors)
• Sodium-Potassium-Exchanging ATPase (metabolism)
• Superoxide Dismutase (metabolism)