Abstract | PURPOSE: The potential for introducing transmissible spongiform encephalopathy (TSE) into islet cells was indicated by recognizing that Liberase HI is isolated from Clostridium histolyticum grown in media containing brain-heart infusion broth. A national team within the Japanese Pancreas and Islet Transplantation Association implemented an islet transplantation program in Japan using Liberase HI. The program comprised 65 islet isolations from non-heart-beating donors and 34 transplants into 18 patients. Herein, we have summarized how the Association followed these recipients over the long term. PROCEDURES: MAIN FINDINGS: Western blotting and immunohistochemical staining did not detect prions in Liberase HI. Diffusion MRI and EEG evaluations performed by CJD specialists confirmed that none of the transplanted recipients had CJD. CONCLUSIONS: Three years of follow-up revealed that none of the Japanese recipients of islet transplants developed CJD. Prion bioassays showed that the Liberase HI used to isolate islets for transplantation was free of infectious TSE prions.
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Authors | T Saito, T Anazawa, M Gotoh, S Uemoto, T Kenmochi, Y Kuroda, S Satomi, T Itoh, Y Yasunami, T Kitamoto, S Mohri, S Teraoka |
Journal | Transplantation proceedings
(Transplant Proc)
Vol. 42
Issue 10
Pg. 4213-6
(Dec 2010)
ISSN: 1873-2623 [Electronic] United States |
PMID | 21168667
(Publication Type: Journal Article)
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Copyright | Copyright © 2010 Elsevier Inc. All rights reserved. |
Chemical References |
- Collagenases
- Liberase
- Thermolysin
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Topics |
- Animals
- Blotting, Western
- Collagenases
(administration & dosage)
- Immunohistochemistry
- Islets of Langerhans Transplantation
(adverse effects)
- Japan
- Mice
- Prion Diseases
(transmission)
- Societies, Medical
- Thermolysin
(administration & dosage)
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