[Interleukin-2 reduces calcium handling capacity of rat ventricular myocytes during anoxia/reoxygenation].

Abstract

AIM:

To investigate the effect of interleukin-2(IL-2) on the cell contractility and calcium handling in cardiomyocytes during normoxia or anoxia/reoxygenation.

METHODS:

Chemical anoxia introduced by Krebs-Henseleit(K-H) solution containing 10(-3) mol/L sodium dithionite was used in the enzymatically isolated rat ventricular myocytes. The video-tracking system and spectrofluorometric method were employed to verify the cell contraction and calcium handling of the single myocyte.

RESULTS:

During anoxia, the cell contraction, amplitude of calcium transient induced by electrical stimulation and Ca2+ release induced by caffeine were depressed while resting calcium level was elevated, but the activity of the L-type calcium channels were not changed. All the parameters could not return to the pre-anoxia level during reoxygenation. IL-2 at 2 x 10(5) U/L administrated during anoxia aggravated the effect of reoxygenation on cell contraction and the calcium handling.

CONCLUSION:

Coexistence of IL-2 during anoxia aggravated the effect of reoxygenation on the cell contraction and calcium handling in the isolated rat ventricular myocytes, in which the reduced calcium release from sarcoplasmic reticulum was involved.

• Authors: Chun-mei Cao, Qiang Xia, Zhi-guo Ye, Yue-liang Shen, Jun-zhu Chen
• Journal: Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology (Zhongguo Ying Yong Sheng Li Xue Za Zhi) Vol. 20 Issue 2 Pg. 111-5 (May 2004) ISSN: 1000-6834 [Print] China
• PMID: 21166188 (Publication Type: English Abstract, Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Interleukin-2
• Oxygen
• Calcium

Topics

• Animals
• Calcium (metabolism)
• Cell Hypoxia
• Cells, Cultured
• Heart Ventricles (cytology)
• Interleukin-2 (pharmacology)
• Male
• Myocardial Contraction (drug effects)
• Myocytes, Cardiac (drug effects, metabolism)
• Oxygen (metabolism)
• Rats
• Rats, Sprague-Dawley
• Sarcoplasmic Reticulum (metabolism)