Quantification of meclizine in human plasma by high performance liquid chromatography-mass spectrometry.

Abstract

PURPOSE:

Meclizine is an antihistamine and has been widely used for prophylactic treatment of motion sickness. To facilitate its pharmacokinetic study in human subjects, a high performance liquid chromatography-mass spectrometric method employing positive electrospray ionization was developed for the determination of meclizine concentration in human plasma.

METHODS:

Meclizine together with the internal standard (flunarizine) was extracted from 0.1 ml of human plasma by protein precipitation using acetonitrile. The chromatography was performed using a Zorbax SB-C18 column (150 × 2.1mm, 5 μm, Agilent) with the mobile phase consisting of acetonitrile and 0.2% formic acid containing 2mM amino acetate. Multiple reaction monitoring was used for quantification. The validation of the method including sensitivity, linearity, reproducibility and stability was examined.

RESULTS:

The lower limit of quantification (LLOQ) of the developed assay method for meclizine was 0.5 ng/ml and the linear calibration curve was acquired with R² > 0.99 between 0.5 and 200ng/ml. The intra-day and inter-day variation of the current assay was evaluated with the coefficient of variations (CVs%) within 12.92% at LLOQ and 7.15% for other quality control samples, whereas the mean accuracy ranged from 99.2% to 102.7%. The samples were stable under the storage conditions at least for a month.

CONCLUSION:

The present method provides a robust, fast and sensitive analytical tool for meclizine in human plasma and has been successfully applied to a clinical pharmacokinetic study in 20 subjects.

• Authors: Zhijun Wang, Shuai Qian, Qizhi Zhang, Moses S S Chow
• Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (J Chromatogr B Analyt Technol Biomed Life Sci) Vol. 879 Issue 1 Pg. 95-9 (Jan 01 2011) ISSN: 1873-376X [Electronic] Netherlands
• PMID: 21163711 (Publication Type: Journal Article)
• Copyright: Copyright © 2010 Elsevier B.V. All rights reserved.

Chemical References

• Acetonitriles
• Formates
• formic acid
• Meclizine
• Flunarizine
• acetonitrile

Topics

• Acetonitriles (chemistry)
• Chromatography, High Pressure Liquid (methods)
• Drug Stability
• Flunarizine (analysis)
• Formates (chemistry)
• Humans
• Least-Squares Analysis
• Mass Spectrometry (methods)
• Meclizine (blood, pharmacokinetics)
• Reproducibility of Results
• Sensitivity and Specificity