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HPLC analysis of para-aminosalicylic acid and its metabolite in plasma, cerebrospinal fluid and brain tissues.

Abstract
Para-aminosalicylic acid (PAS), an approved drug for treatment of tuberculosis, is a promising therapeutic agent for treatment of manganese (Mn)-induced parkinsonian syndromes. Lack of a quantifying method, however, has hindered the clinical evaluation of its efficacy and there upon new drug development. This study was aimed at developing a simple and effective method to quantify PAS and its major metabolite, N-acetyl-para-aminosalicylic acid (AcPAS), in plasma, cerebrospinal fluid (CSF) and tissues. Biological samples underwent one-step protein precipitation. The supernatant was fractionated on a reversed-phase C18 column with a gradient mobile system, followed by on-line fluorescence detection. The lower limits of quantification for both PAS and AcPAS were 50 ng/ml of plasma and 17 ng/g of tissues. The intra-day and inter-day precision values did not exceed 5% and 8%, respectively, in all three matrices. The method was used to quantify PAS and AcPAS in rat plasma and brain following a single iv injection of PAS. Data showed a greater amount of PAS than AcPAS in plasma, while a greater amount of AcPAS than PAS was found in brain tissues. The method has been proven to be sensitive, reproducible, and practically useful for laboratory and clinical investigations of PAS in treatment of Mn Parkinsonism.
AuthorsLan Hong, Wendy Jiang, Wei Zheng, Su Zeng
JournalJournal of pharmaceutical and biomedical analysis (J Pharm Biomed Anal) Vol. 54 Issue 5 Pg. 1101-9 (Apr 05 2011) ISSN: 1873-264X [Electronic] England
PMID21159459 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
CopyrightCopyright © 2010 Elsevier B.V. All rights reserved.
Chemical References
  • Aminosalicylic Acid
Topics
  • Aminosalicylic Acid (analysis, blood, cerebrospinal fluid, metabolism)
  • Animals
  • Brain (metabolism)
  • Calibration
  • Chromatography, High Pressure Liquid (methods)
  • Drug Monitoring (methods)
  • Drug Stability
  • Injections, Intravenous
  • Rats
  • Rats, Sprague-Dawley
  • Reference Standards
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Tissue Distribution

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