[Effect of ginkgolides on gene expression of HIF-1alpha in primary cultured neurons].

Abstract

AIM:

To study the effects of ginkgolides (Gin) on the expression of hypoxia inducible factor-1 (HIF-1alpha) in hypoxic/ischemic neurons.

METHODS:

The gene expression of HIF-1alpha pretreated with or without Gin (37.5 microg/ml) was observed by RT-PCR on primary cultured cortical neurons in the condition of hypoxia and oxygen-glucose deprivation.

RESULTS:

Some basic expression of HIF-1alpha mRNA were observed in cultured cortical neurons. The expression of HIF-1alpha mRNA increased after 24 h treatment with Gin. The level of HIF-la mRNA increased also after 1 h hypoxia and further enhanced after the pretreatment with Gin. The expression of HIF-1alpha mRNA decreased with the deprivation of both oxygen and glucose, which reversed after the pretreatment of Gin.

CONCLUSION:

Gin could increase the expression of HIF-1alpha mRNA in hypoxic/ischemic cortical neurons.

• Authors: Li Zhu, Xiao-Mei Wu, Jian-Cheng Li, Shu-Yi Jin
• Journal: Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology (Zhongguo Ying Yong Sheng Li Xue Za Zhi) Vol. 20 Issue 4 Pg. 354-7 (Nov 2004) ISSN: 1000-6834 [Print] China
• PMID: 21158113 (Publication Type: English Abstract, Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Ginkgolides
• Hif1a protein, mouse
• Hypoxia-Inducible Factor 1, alpha Subunit
• RNA, Messenger

Topics

• Animals
• Cell Hypoxia
• Gene Expression
• Ginkgolides (pharmacology)
• Hypoxia-Inducible Factor 1, alpha Subunit (genetics, metabolism)
• Mice
• Mice, Inbred ICR
• Neurons (drug effects, metabolism)
• RNA, Messenger (genetics)