Abstract |
Fibroblasts ( Fibs) contribution to neoplastic progression, tumor growth, angiogenesis, and metastasis has been recently reported by several research groups. In this study it was investigated if fibroblasts are the source of brain-derived neurotrophic factor ( BDNF), which plays a crucial role in the progression of oral squamous cell carcinoma. In a novel in vitro system oral Fibs were cultured with SCC-25 lingual squamous cell carcinoma cells for 7days. Factors related with this interaction were investigated by quantitative PCR and western blot. In the co-culture, fibroblasts were converted to carcinoma-associated fibroblasts (CAFs), which in return initiated epithelial-mesenchymal transition (EMT) of SCC-25 cells. The induced CAFs produced increased levels of BDNF, which interacted with the increased-expressed neurothrophin receptor B (TrkB) on EMT-converted SCC-25 cells. Possible regulatory factors of BDNF expression ( tumor necrosis factor-α and interleukin-1-β) were detected both in CAFs and EMT- tumor cells. In CAFs: IL-1β-, in SCC-25 cells TNF-α-gene-expression was significantly increased in co-culture conditions. Activated fibroblasts (CAFs) and mesenchymal transitioned tumor cells might use the BDNF-TrkB axis and its regulation to harmonize their interaction in the process of tumor progression.
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Authors | József Dudás, Mario Bitsche, Volker Schartinger, Christina Falkeis, Georg Mathias Sprinzl, Herbert Riechelmann |
Journal | Oral oncology
(Oral Oncol)
Vol. 47
Issue 2
Pg. 98-103
(Feb 2011)
ISSN: 1879-0593 [Electronic] England |
PMID | 21147546
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2010 Elsevier Ltd. All rights reserved. |
Chemical References |
- Brain-Derived Neurotrophic Factor
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Topics |
- Animals
- Blotting, Western
- Brain-Derived Neurotrophic Factor
(metabolism)
- Carcinoma, Squamous Cell
(genetics, metabolism, pathology)
- Cell Line, Tumor
- Epithelial-Mesenchymal Transition
(genetics, physiology)
- Fibroblasts
(metabolism, pathology, physiology)
- Gene Expression Regulation, Neoplastic
(genetics)
- Humans
- Mouth Neoplasms
(genetics, metabolism, pathology)
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