[Roles of NRF2 regulating gamma-glutamylcysteine synthetase in lung of rats with chronic obstructive pulmonary disease].

Abstract

AIM:

To investigate the expression and relationship of gamma-glutamylcysteine synthetase (gamma-GCS) and NF-E2-related factor2 (NRR2) in lung of rat with chronic obstructive pulmonary disease (COPD)in order to elucidate the possible important role of gamma-GCS and NRF2 in pathogenesis of COPD.

METHODS:

The rat COPD model was established by intratracheal instillation of lipopolysaccharide twice and exposed to cigarette smoke daily. The gamma-GCS activity was measured, the expression of gamma-GCS mRNA in lung was examined by in situ hybridization (ISH) and reverse transcription-polymerase chain reaction (RT-PCR), the protein expressions of NRF2, gamma-GCS in lung were detected by immunohistochemical (IH) and Western blot respectively.

RESULTS:

The gamma-GCS activity was higher in COPD group than that in control group. The expressions of gamma-GCS mRNA in COPD group was stronger than those in control group. ISH showed that the gamma-GCS mRNA was expressed in alveolar epithelium and bronchial smooth muscle cell in COPD. The protein expressions of NRF2, gamma-GCS were significantly higher than the control group. IH showed that NRF2, gamma-GCS proteins were expressed in alveolar and bronchial epithelium in the COPD group. There was a positive correlation between NRF2 and gamma-GCS and gamma-GCS mRNA.

CONCLUSION:

NRF2 may play an important role in the mechanism of COPD oxidative stress vis up-regulation of gamma-GCS.

• Authors: Lin Chen, Ai-Guo Dai, Rui-Cheng Hu
• Journal: Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology (Zhongguo Ying Yong Sheng Li Xue Za Zhi) Vol. 24 Issue 3 Pg. 339-42 (Aug 2008) ISSN: 1000-6834 [Print] China
• PMID: 21141598 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• NF-E2-Related Factor 2
• Nfe2l2 protein, rat
• Glutamate-Cysteine Ligase

Topics

• Animals
• Glutamate-Cysteine Ligase (metabolism)
• Lung (metabolism)
• Male
• NF-E2-Related Factor 2 (metabolism)
• Oxidative Stress
• Pulmonary Disease, Chronic Obstructive (metabolism, physiopathology)
• Random Allocation
• Rats
• Rats, Wistar