Modulation of NADPH oxidase activation in cerebral ischemia/reperfusion injury in rats.

Abstract

NADPH oxidase is a major complex that produces reactive oxygen species (ROSs) during the ischemic period and aggravates brain damage and cell death after ischemic injury. Although many approaches have been tested for preventing production of ROSs by NADPH oxidase in ischemic brain injury, the regulatory mechanisms of NADPH oxidase activity after cerebral ischemia are still unclear. The aim of this study is identifying apocynin as a critical modulator of NADPH oxidase and elucidating its role as a neuroprotectant in an experimental model of brain ischemia in rat. Treatment of apocynin 5min before of reperfusion attenuated cerebral ischemia in rats. Administration of apocynin showed marked reduction in infarct size compared with that of control rats. Medial carotid artery occlusion (MCAo)-induced cerebral ischemia was also associated with an increase in, nitrotyrosine formation, as well as IL-1β expression, IκB degradation and ICAM expression in ischemic regions. These expressions were markedly inhibited by the treatment of apocynin. We also demonstrated that apocynin reduces levels of apoptosis (TUNEL, Bax and Bcl-2 expression) resulting in a reduction in the infarct volume in ischemia-reperfusion brain injury. This new understanding of apocynin induced adaptation to ischemic stress and inflammation could suggest novel avenues for clinical intervention during ischemic and inflammatory diseases.

• Authors: Tiziana Genovese, Emanuela Mazzon, Irene Paterniti, Emanuela Esposito, Placido Bramanti, Salvatore Cuzzocrea
• Journal: Brain research (Brain Res) Vol. 1372 Pg. 92-102 (Feb 04 2011) ISSN: 1872-6240 [Electronic] Netherlands
• PMID: 21138737 (Publication Type: Journal Article)
• Copyright: Copyright © 2010 Elsevier B.V. All rights reserved.

Chemical References

• Acetophenones
• Bax protein, rat
• Enzyme Inhibitors
• I-kappa B Proteins
• Interleukin-1beta
• Peptide Fragments
• Proto-Oncogene Proteins c-bcl-2
• bcl-2-Associated X Protein
• interleukin-1beta (163-171)
• Intercellular Adhesion Molecule-1
• 3-nitrotyrosine
• Tyrosine
• Cytochromes c
• acetovanillone
• NADPH Oxidases

Topics

• Acetophenones (therapeutic use)
• Animals
• Apoptosis (drug effects)
• Brain (drug effects, pathology)
• Cytochromes c (metabolism)
• Disease Models, Animal
• Enzyme Inhibitors (therapeutic use)
• Gene Expression Regulation, Enzymologic (drug effects)
• I-kappa B Proteins (metabolism)
• In Situ Nick-End Labeling (methods)
• Infarction, Middle Cerebral Artery (drug therapy, enzymology, pathology)
• Intercellular Adhesion Molecule-1 (metabolism)
• Interleukin-1beta (metabolism)
• Male
• NADPH Oxidases (metabolism)
• Neurologic Examination
• Peptide Fragments (metabolism)
• Proto-Oncogene Proteins c-bcl-2 (metabolism)
• Rats
• Rats, Wistar
• Reperfusion Injury (drug therapy, enzymology)
• Tyrosine (analogs & derivatives, metabolism)
• bcl-2-Associated X Protein (metabolism)