Efficient conjugation doxorubicin to bacterial magnetic nanoparticles via a triplex hands coupling reagent.

Abstract

By using a novel triplex "hand" coupling reagent, tris-succinimidyl aminotriacetate (TSAT), doxorubicin loading efficiency of BMP can be greatly enhanced in comparison with that of dual functional linkers. The drug loading ratio of doxorubicin-coupled bacterial magnetic nanoparticles produced by this method (DBMP-T) is 62%. DBMP-T disperse well, have a narrow size distribution, exhibit a high magnetic response, is sensitive to pH to facilitate drug release and are strongly cytotoxic to HePG2 and MCF-7 cancer cells.

• Authors: Lin Guo, Ji Huang, Li-Min Zheng
• Journal: Journal of nanoscience and nanotechnology (J Nanosci Nanotechnol) Vol. 10 Issue 10 Pg. 6514-9 (Oct 2010) ISSN: 1533-4880 [Print] United States
• PMID: 21137755 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Magnetite Nanoparticles
• Succinimides
• tris-succinimidyl aminotriacetate
• Doxorubicin
• Glutaral

Topics

• Carcinoma, Hepatocellular (drug therapy, metabolism)
• Cell Line, Tumor
• Cell Survival (drug effects)
• Doxorubicin (administration & dosage, chemistry, pharmacokinetics)
• Glutaral (chemistry)
• Hep G2 Cells
• Humans
• Hydrogen-Ion Concentration
• Light
• Liver Neoplasms (drug therapy, metabolism)
• Magnetite Nanoparticles (chemistry)
• Magnetospirillum (chemistry)
• Microscopy, Electron, Transmission
• Microscopy, Fluorescence
• Particle Size
• Scattering, Radiation
• Spectrophotometry, Infrared
• Succinimides (chemistry)