(Coixan polysaccharide)-graft-polyethylenimine folate for tumor-targeted gene delivery.

Abstract

CP-PEI-FA was prepared as an effective vector for in vitro and in vivo tumor-targeted gene delivery. The structures of the polymers were characterized, and their DNA condensation capability, particle sizes, zeta potentials, cytotoxicity and in vitro/in vivo transfection were examined. The cytotoxicity of CP-PEI-FA was significantly lower than that of PEI 25 kDa and close to that of PEI 1200. The in vitro transfection of CP-PEI-FA was tested in C6 and HeLa cells (FR-positive cells) and A549 cells (FR-negative cells). CP-PEI-FA showed a high targeting specificity and good gene transfection efficiency in FR-positive cells. These results indicate that CP-PEI-FA is a safe and effective polyplex-forming agent for both in vitro and in vivo transfection of plasmid DNA.

• Authors: Qiying Jiang, Peihua Shi, Chunyan Li, Qingqing Wang, Fujian Xu, Wantai Yang, Guping Tang
• Journal: Macromolecular bioscience (Macromol Biosci) Vol. 11 Issue 3 Pg. 435-44 (Mar 10 2011) ISSN: 1616-5195 [Electronic] Germany
• PMID: 21136538 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Polysaccharides
• coixan
• Polyethyleneimine
• DNA
• Folic Acid

Topics

• Animals
• Cell Death (drug effects)
• Cell Line, Tumor
• DNA (genetics)
• Electrophoretic Mobility Shift Assay
• Folic Acid (chemical synthesis, chemistry, toxicity)
• Gene Transfer Techniques
• Humans
• Magnetic Resonance Spectroscopy
• Mice
• Mice, Inbred BALB C
• Microscopy, Fluorescence
• Neoplasms (metabolism, pathology)
• Photoelectron Spectroscopy
• Plasmids (genetics)
• Polyethyleneimine (chemical synthesis, chemistry, toxicity)
• Polysaccharides (chemical synthesis, chemistry, toxicity)
• Rats
• Spectrophotometry, Ultraviolet
• Transfection