The initial mechanical tissue disruption of
spinal cord injury (SCI) is followed by a period of secondary injury that increases the size of the lesion. Secondary
injuries are associated with
edema,
inflammation, excessive
cytokine release, excitotoxicity and cell apoptosis. 3,4-dihydroxyphenyl
lactic acid (DLA) is one of the major water-soluble components of chemical constituents from Salvia miltiorrhiza (SM). To investigate the inhibition effects of DLA on secondary injury of SCI, focusing especially on suppression of inflammatory responses and the mechanism of this effect, the following studies were performed: Basso, Beattie, and Bresnahan (BBB) scores to assess motor functions till 10 days after SCI; Nissl and
Fast Blue histological staining and immunohistochemistry of inhibitory-kappa B-alpha (IκB-α) and
nuclear factor-kappa B (NF-κB) p65
subunit protein; levels of
myeloperoxidase (MPO) activity analysis as an
indicator of polymorphonuclear infiltration;
IL-6 production in plasma 10 days after SCI; Western blot analysis to determine cytoplasm levels of IκB-α and NF-κB p65 subunit
proteins in the nuclear fractions 10 days after SCI. DLA significantly attenuated the motor function and tissue damage following SCI in rats, significant reduced polymorphonuclear cell infiltration and
IL-6 production, as well as reduced cytoplasm IκB-α degradation and the nuclear translocation of NF-κB p65
subunit protein after SCI. In conclusion, the results clearly demonstrate that DLA inhibit the
inflammation responses induced by SCI via inhibiting effect of production of
IL-6 and nuclear translocation of NF-κB.