Abstract |
In this study, the adjuvanticity of methylvinylether-co- maleic anhydride ( Gantrez(®)AN) nanoparticles (NP) was investigated in an oral immunisation experiment of pigs against F4+enterotoxigenic Escherichia coli (F4+ETEC). In addition, Wheat Germ Agglutinin (WGA)-coating of the nanoparticles was tested for enterocyte-targeting. Pigs were either vaccinated with F4 fimbriae, F4 encapsulated in Gantrez(®)AN NP, F4 encapsulated in Gantrez(®)AN NP coated with WGA or F4 fimbriae mixed with empty Gantrez(®)AN NP. Only vaccination with the combination of F4 mixed with empty Gantrez(®)AN NP improved protection against F4+ETEC infection. In addition, vaccination with this formulation also resulted in an F4-specific serum antibody response prior to F4+ETEC challenge. Encapsulation of F4 in Gantrez(®)AN NP only raised the serum antibody response after F4+ETEC challenge compared to soluble F4, but did not improve protection, whereas WGA-coating almost completely abolished the serum antibody response. These data indicate that nanoparticle effects after F4 encapsulation were of lesser importance for the adjuvant effect of Gantrez(®)AN NP, contrarily to the reactivity of the Gantrez(®)AN polymer used to prepare the nanoparticles.
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Authors | Katrien Vandamme, Vesna Melkebeek, Melkebeek Vesna, Eric Cox, Cox Eric, Jean Paul Remon, Remon Jean Paul, Chris Vervaet, Vervaet Chris |
Journal | Veterinary immunology and immunopathology
(Vet Immunol Immunopathol)
Vol. 139
Issue 2-4
Pg. 148-55
(Feb 15 2011)
ISSN: 1873-2534 [Electronic] Netherlands |
PMID | 21130503
(Publication Type: Controlled Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2010 Elsevier B.V. All rights reserved. |
Chemical References |
- Adjuvants, Immunologic
- Bacterial Vaccines
- Maleates
- Polyvinyls
- Wheat Germ Agglutinins
- polyvinylmethoxyethylene-maleic anhydride copolymer
- Fimbriae Proteins
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Topics |
- Adjuvants, Immunologic
(administration & dosage)
- Administration, Oral
- Animals
- Bacterial Shedding
- Bacterial Vaccines
(administration & dosage, immunology)
- Enterotoxigenic Escherichia coli
(immunology)
- Escherichia coli Infections
(microbiology, prevention & control, veterinary)
- Feces
(microbiology)
- Fimbriae Proteins
(immunology)
- Maleates
(immunology)
- Nanoparticles
- Polyvinyls
- Swine
- Swine Diseases
(prevention & control)
- Wheat Germ Agglutinins
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