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Adjuvant effect of Gantrez®AN nanoparticles during oral vaccination of piglets against F4+enterotoxigenic Escherichia coli.

Abstract
In this study, the adjuvanticity of methylvinylether-co-maleic anhydride (Gantrez(®)AN) nanoparticles (NP) was investigated in an oral immunisation experiment of pigs against F4+enterotoxigenic Escherichia coli (F4+ETEC). In addition, Wheat Germ Agglutinin (WGA)-coating of the nanoparticles was tested for enterocyte-targeting. Pigs were either vaccinated with F4 fimbriae, F4 encapsulated in Gantrez(®)AN NP, F4 encapsulated in Gantrez(®)AN NP coated with WGA or F4 fimbriae mixed with empty Gantrez(®)AN NP. Only vaccination with the combination of F4 mixed with empty Gantrez(®)AN NP improved protection against F4+ETEC infection. In addition, vaccination with this formulation also resulted in an F4-specific serum antibody response prior to F4+ETEC challenge. Encapsulation of F4 in Gantrez(®)AN NP only raised the serum antibody response after F4+ETEC challenge compared to soluble F4, but did not improve protection, whereas WGA-coating almost completely abolished the serum antibody response. These data indicate that nanoparticle effects after F4 encapsulation were of lesser importance for the adjuvant effect of Gantrez(®)AN NP, contrarily to the reactivity of the Gantrez(®)AN polymer used to prepare the nanoparticles.
AuthorsKatrien Vandamme, Vesna Melkebeek, Melkebeek Vesna, Eric Cox, Cox Eric, Jean Paul Remon, Remon Jean Paul, Chris Vervaet, Vervaet Chris
JournalVeterinary immunology and immunopathology (Vet Immunol Immunopathol) Vol. 139 Issue 2-4 Pg. 148-55 (Feb 15 2011) ISSN: 1873-2534 [Electronic] Netherlands
PMID21130503 (Publication Type: Controlled Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2010 Elsevier B.V. All rights reserved.
Chemical References
  • Adjuvants, Immunologic
  • Bacterial Vaccines
  • Maleates
  • Polyvinyls
  • Wheat Germ Agglutinins
  • polyvinylmethoxyethylene-maleic anhydride copolymer
  • Fimbriae Proteins
Topics
  • Adjuvants, Immunologic (administration & dosage)
  • Administration, Oral
  • Animals
  • Bacterial Shedding
  • Bacterial Vaccines (administration & dosage, immunology)
  • Enterotoxigenic Escherichia coli (immunology)
  • Escherichia coli Infections (microbiology, prevention & control, veterinary)
  • Feces (microbiology)
  • Fimbriae Proteins (immunology)
  • Maleates (immunology)
  • Nanoparticles
  • Polyvinyls
  • Swine
  • Swine Diseases (prevention & control)
  • Wheat Germ Agglutinins

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